During the extended prophase of Drosophila gametogenesis, spermatocytes undergo robust gene transcription and store many transcripts in the cytoplasm in a repressed state, until translational activation of select mRNAs in later steps of spermatogenesis. Here, we characterize the Drosophila Doublefault (Dbf) protein as a C2H2 zinc-finger protein, primarily expressed in testes, that is required for normal meiotic division and spermiogenesis. Loss of Dbf causes premature centriole disengagement and affects spindle structure, chromosome segregation and cytokinesis. We show that Dbf interacts with the RNA-binding protein Syncrip/hnRNPQ, a key regulator of localized translation in Drosophila We propose that the pleiotropic effects of dbf loss-of-function mutants are associated with the requirement of dbf function for translation of specific transcripts in spermatocytes. In agreement with this hypothesis, Dbf protein binds cyclin B mRNA and is essential for translation of cyclin B in mature spermatocytes.

Drosophila doublefault protein coordinates multiple events during male meiosis by controlling mRNA translation / Sechi, S.; Frappaolo, A.; Karimpour-Ghahnavieh, A.; Gottardo, M.; Burla, R.; Di Francesco, L.; Szafer-Glusman, E.; Schinina, E.; Fuller, M. T.; Saggio, I.; Riparbelli, M. G.; Callaini, G.; Giansanti, M. G.. - In: DEVELOPMENT. - ISSN 1477-9129. - 146:22(2019). [10.1242/dev.183053]

Drosophila doublefault protein coordinates multiple events during male meiosis by controlling mRNA translation

Frappaolo A.;Karimpour-Ghahnavieh A.;Gottardo M.;Burla R.;Schinina E.;Saggio I.;Giansanti M. G.
2019

Abstract

During the extended prophase of Drosophila gametogenesis, spermatocytes undergo robust gene transcription and store many transcripts in the cytoplasm in a repressed state, until translational activation of select mRNAs in later steps of spermatogenesis. Here, we characterize the Drosophila Doublefault (Dbf) protein as a C2H2 zinc-finger protein, primarily expressed in testes, that is required for normal meiotic division and spermiogenesis. Loss of Dbf causes premature centriole disengagement and affects spindle structure, chromosome segregation and cytokinesis. We show that Dbf interacts with the RNA-binding protein Syncrip/hnRNPQ, a key regulator of localized translation in Drosophila We propose that the pleiotropic effects of dbf loss-of-function mutants are associated with the requirement of dbf function for translation of specific transcripts in spermatocytes. In agreement with this hypothesis, Dbf protein binds cyclin B mRNA and is essential for translation of cyclin B in mature spermatocytes.
2019
centrosome; cytokinesis; drosophila; male meiosis; spermatogenesis
01 Pubblicazione su rivista::01a Articolo in rivista
Drosophila doublefault protein coordinates multiple events during male meiosis by controlling mRNA translation / Sechi, S.; Frappaolo, A.; Karimpour-Ghahnavieh, A.; Gottardo, M.; Burla, R.; Di Francesco, L.; Szafer-Glusman, E.; Schinina, E.; Fuller, M. T.; Saggio, I.; Riparbelli, M. G.; Callaini, G.; Giansanti, M. G.. - In: DEVELOPMENT. - ISSN 1477-9129. - 146:22(2019). [10.1242/dev.183053]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1334563
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