We report the fabrication of silicon chips containing a row of 667 pillars, 10 by 20 mm in cross-section, etched to a depth of 80 mm with adjacent pillars being separated by 3.5 mm. The chips were used to separate white blood cells from whole blood in less than 2 min and for subsequent PCR of a genomic target (eNOS). Chip fluid dynamics were validated experimentally using CoventorWare TM microfluidic simulation software. The amplicon concentrations were determined using microchip capillary electrophoresis and were >40% of that observed in conventional PCR tubes for chips with and without pillars. Reproducible on-chip PCR was achieved using white blood cell preparations isolated from whole human blood pumped through the chip.
Micropillar array chip for integrated white blood cell isolation and PCR / Nicholas J., Panaro; Xing Jian, Lou; Fortina, Paolo; Larry J., Kricka; Peter, Wilding. - In: BIOMOLECULAR ENGINEERING. - ISSN 1389-0344. - STAMPA. - 21:6(2005), pp. 157-162. [10.1016/j.bioeng.2004.11.001]
Micropillar array chip for integrated white blood cell isolation and PCR
FORTINA, PAOLO;
2005
Abstract
We report the fabrication of silicon chips containing a row of 667 pillars, 10 by 20 mm in cross-section, etched to a depth of 80 mm with adjacent pillars being separated by 3.5 mm. The chips were used to separate white blood cells from whole blood in less than 2 min and for subsequent PCR of a genomic target (eNOS). Chip fluid dynamics were validated experimentally using CoventorWare TM microfluidic simulation software. The amplicon concentrations were determined using microchip capillary electrophoresis and were >40% of that observed in conventional PCR tubes for chips with and without pillars. Reproducible on-chip PCR was achieved using white blood cell preparations isolated from whole human blood pumped through the chip.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
