Abstract Background: A wide debate is ongoing regarding the role of cutaneous dysbiosis in the pathogenesis and evolution of difficult-to-treat chronic wounds. Nowadays, probiotic treatment considered as an useful tool to counteract dysbio- sis but the evidence in regard to their therapeutic use in the setting of difficult-to-treat cutaneous ulcers is still poor. Aim: clinical report: An 83-year-old woman suffering a critical limb ischemia and an infected difficult-to-treat ulcer- ated cutaneous lesion of the right leg, was complementary treated with local application of a mixture of probiotic bacteria. Methods: Microbiological and metabolomic analysis were conducted on wound swabs obtained before and after bacteriotherapy. Results: During the treatment course, a progressive healing of the lesion was observed with microbiological resolution of the polymicrobial infection of the wound. Metabolomic analysis showed a significant difference in the local concentration of propionate, 2-hydroxyisovalerate, 2-oxoisocaproate, 2,3-butanediol, putrescine, thymine, and trimethylamine before and after bacteriotherapy. Conclusion: The microbiological and metabolomic results seem to confirm the usefulness of complementary pro- biotic treatment in difficult-to-treat infected wounds. Further investigations are needed to confirm these preliminary findings.

Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: a novel tailored treatment strategy / Salvatore, Venosi; Ceccarelli, Giancarlo; DE ANGELIS, Massimiliano; Luca, Laghi; Bianchi, Laura; Martinelli, Ombretta; Maruca, Debora; Cavallari, EUGENIO NELSON; Fabrizia, Toscanella; Vassalini, Paolo; Trinchieri, Vito; Oliva, Alessandra; D'Ettorre, Gabriella. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 17:1(2019), pp. 1-9. [10.1186/s12967-019-2111-0]

Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: a novel tailored treatment strategy

Giancarlo Ceccarelli
;
Massimiliano de Angelis;BIANCHI, Laura;Ombretta Martinelli;Debora Maruca;Eugenio Nelson Cavallari;VASSALINI, PAOLO;Vito Trinchieri;Alessandra Oliva;Gabriella d’Ettorre
2019

Abstract

Abstract Background: A wide debate is ongoing regarding the role of cutaneous dysbiosis in the pathogenesis and evolution of difficult-to-treat chronic wounds. Nowadays, probiotic treatment considered as an useful tool to counteract dysbio- sis but the evidence in regard to their therapeutic use in the setting of difficult-to-treat cutaneous ulcers is still poor. Aim: clinical report: An 83-year-old woman suffering a critical limb ischemia and an infected difficult-to-treat ulcer- ated cutaneous lesion of the right leg, was complementary treated with local application of a mixture of probiotic bacteria. Methods: Microbiological and metabolomic analysis were conducted on wound swabs obtained before and after bacteriotherapy. Results: During the treatment course, a progressive healing of the lesion was observed with microbiological resolution of the polymicrobial infection of the wound. Metabolomic analysis showed a significant difference in the local concentration of propionate, 2-hydroxyisovalerate, 2-oxoisocaproate, 2,3-butanediol, putrescine, thymine, and trimethylamine before and after bacteriotherapy. Conclusion: The microbiological and metabolomic results seem to confirm the usefulness of complementary pro- biotic treatment in difficult-to-treat infected wounds. Further investigations are needed to confirm these preliminary findings.
2019
Bacteriotherapy; topical probiotic; metabolomic; wound; wound care; wound healing; antimicrobial resistance
01 Pubblicazione su rivista::01a Articolo in rivista
Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: a novel tailored treatment strategy / Salvatore, Venosi; Ceccarelli, Giancarlo; DE ANGELIS, Massimiliano; Luca, Laghi; Bianchi, Laura; Martinelli, Ombretta; Maruca, Debora; Cavallari, EUGENIO NELSON; Fabrizia, Toscanella; Vassalini, Paolo; Trinchieri, Vito; Oliva, Alessandra; D'Ettorre, Gabriella. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 17:1(2019), pp. 1-9. [10.1186/s12967-019-2111-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1327860
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