Background and Objective: Bacteria that cause infection of vascular prosthetic grafts produce an exopolysaccharide matrix known as biofilm. Growth in biofilms protects the bacteria from leukocytes, antibodies and antimicrobial drugs. Laser-generated shock waves (SW) can disrupt biofilms and increase drug penetration. This study investigates the possibility of increasing antibiotic delivery and sterilization of vascular prosthetic graft. Study Design/Materials and Methods: Strains of Staphylococcus epidermidis and S. aureus were isolated from infected prosthetic grafts obtained directly from patients. Dacron grafts were inoculated with the isolated bacteria, which were allowed to form adherent bacterial colonies. The colonized grafts underwent the following treatments: (a) antibiotic (vancomycin) alone; (b) antibiotic and SW (c) saline only; and (d) saline and SW. Six hours after treatment, the grafts were sonicated, the effluent was cultured and the colony forming units (CFU) were counted. Results: CFU recovered from control grafts colonized by S. epidermidis were comparable: saline, 3.05108 and saline SW 3.31108. The number of S. epidermidis CFU diminished to 7.61106 after antibiotic treatment but the combined antibiotic SW treatment synergistically decreased CFU number to 1.27104 (P<0.001). S. aureus showed a higher susceptibility to the antibiotic: 2.26106 CFU; antibiotic SW treatment also had an incremental effect: 8.27104 CFU (P<0.001). Conclusions: This study demonstrates that laser-generated shock waves have no effects alone, but can enhance the effectiveness of antibiotics against bacteria associated with prosthetic vascular graft biofilms, suggesting that this treatment may be of value as adjunctive therapy for prosthetic graft infections. Lasers Surg. Med. 29:448–454, 2001. ß 2001 Wiley-Liss, Inc.

Laser-Induced Shock Waves Enhance Sterilization of Infected Vascular Prosthetic Grafts / Nigri, Giuseppe; Tsai, S.; Kossodo, S.; Waterman, P.; Fungaloi, P.; Hooper, D.; Doukas, A.; Lamuraglia, G.. - In: LASERS IN SURGERY AND MEDICINE. - ISSN 0196-8092. - STAMPA. - 29:(2001), pp. 448-454.

Laser-Induced Shock Waves Enhance Sterilization of Infected Vascular Prosthetic Grafts

NIGRI, Giuseppe;
2001

Abstract

Background and Objective: Bacteria that cause infection of vascular prosthetic grafts produce an exopolysaccharide matrix known as biofilm. Growth in biofilms protects the bacteria from leukocytes, antibodies and antimicrobial drugs. Laser-generated shock waves (SW) can disrupt biofilms and increase drug penetration. This study investigates the possibility of increasing antibiotic delivery and sterilization of vascular prosthetic graft. Study Design/Materials and Methods: Strains of Staphylococcus epidermidis and S. aureus were isolated from infected prosthetic grafts obtained directly from patients. Dacron grafts were inoculated with the isolated bacteria, which were allowed to form adherent bacterial colonies. The colonized grafts underwent the following treatments: (a) antibiotic (vancomycin) alone; (b) antibiotic and SW (c) saline only; and (d) saline and SW. Six hours after treatment, the grafts were sonicated, the effluent was cultured and the colony forming units (CFU) were counted. Results: CFU recovered from control grafts colonized by S. epidermidis were comparable: saline, 3.05108 and saline SW 3.31108. The number of S. epidermidis CFU diminished to 7.61106 after antibiotic treatment but the combined antibiotic SW treatment synergistically decreased CFU number to 1.27104 (P<0.001). S. aureus showed a higher susceptibility to the antibiotic: 2.26106 CFU; antibiotic SW treatment also had an incremental effect: 8.27104 CFU (P<0.001). Conclusions: This study demonstrates that laser-generated shock waves have no effects alone, but can enhance the effectiveness of antibiotics against bacteria associated with prosthetic vascular graft biofilms, suggesting that this treatment may be of value as adjunctive therapy for prosthetic graft infections. Lasers Surg. Med. 29:448–454, 2001. ß 2001 Wiley-Liss, Inc.
2001
01 Pubblicazione su rivista::01a Articolo in rivista
Laser-Induced Shock Waves Enhance Sterilization of Infected Vascular Prosthetic Grafts / Nigri, Giuseppe; Tsai, S.; Kossodo, S.; Waterman, P.; Fungaloi, P.; Hooper, D.; Doukas, A.; Lamuraglia, G.. - In: LASERS IN SURGERY AND MEDICINE. - ISSN 0196-8092. - STAMPA. - 29:(2001), pp. 448-454.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/132637
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