The combination of fiber-optic–based platforms for biosensing with nanotechnologies is opening up the chance for the development of in situ, portable, lightweight, versatile, reliable and high-performance optical sensing devices. The route consists of the generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick absorbing metaloxide films on special geometric-modified fibers. This allows measuring precisely and accurately the changes in surface refractive index due to the binding interaction between a biological recognition element and the analyte, with very high sensitivity compared to other optical technology platforms, such as fiber gratings or surface plasmon resonance. The proposed methodology, mixed with the use of specialty fiber structures such as D-shaped fibers, allows improving the light-matter interaction in a strong way. The shift of the LMR has been used to monitor in real-time the biomolecule interactions thanks to a conventional wavelength-interrogation system and an ad-hoc developed microfluidics. A big leap in performance has been attained by detecting femtomolar concentrations in real samples of human serum. The biosensor regeneration has been also studied by using a solution of sodium dodecyl sulphate (SDS), proving the device reusability. Therefore, this technology possibly represents a paradigm shift in the development of a simple, high-specificity and label-free biosensing platform, which can be applied to speed up diagnostic healthcare processes of different diseases toward an early diagnostic and personalized treatment system.

Fiber-optics: a new route towards ultra-low detection limit label-free biosensing / Zubiate, Pablo; Santano, Desiree; Urrutia, Aitor; Zamarreño, Carlos R.; Díaz, Silvia; Matias, Ignacio R.; Arregui, Francisco J.; Giannetti, Ambra; Baldini, Francesco; Fernández-Irigoyen, Joaquín; Santamaría, Enrique; Sinibaldi, Alberto; Michelotti, Francesco; Janner, Davide; Guo, Tuan; Del Villar, Ignacio; Chiavaioli, Francesco. - 11199:(2019). ((Intervento presentato al convegno Seventh European Workshop on Optical Fibre Sensors tenutosi a Limassol, Cyprus [10.1117/12.2540008].

Fiber-optics: a new route towards ultra-low detection limit label-free biosensing

Sinibaldi, Alberto;Michelotti, Francesco;
2019

Abstract

The combination of fiber-optic–based platforms for biosensing with nanotechnologies is opening up the chance for the development of in situ, portable, lightweight, versatile, reliable and high-performance optical sensing devices. The route consists of the generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick absorbing metaloxide films on special geometric-modified fibers. This allows measuring precisely and accurately the changes in surface refractive index due to the binding interaction between a biological recognition element and the analyte, with very high sensitivity compared to other optical technology platforms, such as fiber gratings or surface plasmon resonance. The proposed methodology, mixed with the use of specialty fiber structures such as D-shaped fibers, allows improving the light-matter interaction in a strong way. The shift of the LMR has been used to monitor in real-time the biomolecule interactions thanks to a conventional wavelength-interrogation system and an ad-hoc developed microfluidics. A big leap in performance has been attained by detecting femtomolar concentrations in real samples of human serum. The biosensor regeneration has been also studied by using a solution of sodium dodecyl sulphate (SDS), proving the device reusability. Therefore, this technology possibly represents a paradigm shift in the development of a simple, high-specificity and label-free biosensing platform, which can be applied to speed up diagnostic healthcare processes of different diseases toward an early diagnostic and personalized treatment system.
Seventh European Workshop on Optical Fibre Sensors
Fiber-optic sensing platform, lossy mode resonance, absorbing metal-oxide nanomaterial, integrated microfluidics, femtomolar concentration
04 Pubblicazione in atti di convegno::04b Atto di convegno in volume
Fiber-optics: a new route towards ultra-low detection limit label-free biosensing / Zubiate, Pablo; Santano, Desiree; Urrutia, Aitor; Zamarreño, Carlos R.; Díaz, Silvia; Matias, Ignacio R.; Arregui, Francisco J.; Giannetti, Ambra; Baldini, Francesco; Fernández-Irigoyen, Joaquín; Santamaría, Enrique; Sinibaldi, Alberto; Michelotti, Francesco; Janner, Davide; Guo, Tuan; Del Villar, Ignacio; Chiavaioli, Francesco. - 11199:(2019). ((Intervento presentato al convegno Seventh European Workshop on Optical Fibre Sensors tenutosi a Limassol, Cyprus [10.1117/12.2540008].
File allegati a questo prodotto
File Dimensione Formato  
paper128_SPIE_2019_Fibre sensing protein tau.pdf

solo gestori archivio

Tipologia: Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza: Creative commons
Dimensione 502.41 kB
Formato Adobe PDF
502.41 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1317028
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 0
social impact