In order to investigate the transactivational function of HBV truncated preS/S proteins we have constructed two sets of plasmids and have tested their transactivational potential on the c-myc regulatory sequences and the TPA-responsive element. We found that preS/S proteins only become transactivationally active when truncated at the carboxy terminal end. Furthermore, using immunofluorescence microscopy we determined that the proteins are located exclusively in the cytoplasm, apparently ruling out DNA binding and activation of factors in the nucleus.
Truncated pre-S/S proteins transactivate multiple target sequences / G., Natoli; C., Balsano; M. L., Avantaggiati; E., De Marzio; Artini, Marco; D., Collepardo; E., Elfassi; Levrero, Massimo. - In: ARCHIVES OF VIROLOGY. SUPPLEMENTUM. - ISSN 0939-1983. - 4:(1992), pp. 65-69. (Intervento presentato al convegno Second International Conference: "Current trends in chronically evolving viral hepatitis" tenutosi a Siena nel 24-27 ott. 1990).
Truncated pre-S/S proteins transactivate multiple target sequences.
ARTINI, Marco;LEVRERO, Massimo
1992
Abstract
In order to investigate the transactivational function of HBV truncated preS/S proteins we have constructed two sets of plasmids and have tested their transactivational potential on the c-myc regulatory sequences and the TPA-responsive element. We found that preS/S proteins only become transactivationally active when truncated at the carboxy terminal end. Furthermore, using immunofluorescence microscopy we determined that the proteins are located exclusively in the cytoplasm, apparently ruling out DNA binding and activation of factors in the nucleus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
