In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.

Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells / Macone, A.; Masciarelli, S.; Palombarini, Federica; Quaglio, D.; Boffi, A.; Trabuco, M. C.; Baiocco, P.; Fazi, F.; Bonamore, A.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 9:1(2019), p. 11749. [10.1038/s41598-019-48037-z]

Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells

Macone A.
;
Masciarelli S.;PALOMBARINI, FEDERICA;Quaglio D.;Boffi A.;Baiocco P.;Fazi F.;Bonamore A.
2019

Abstract

In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
2019
human ferritin; cytochrome c; myeloid leukemia
01 Pubblicazione su rivista::01a Articolo in rivista
Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells / Macone, A.; Masciarelli, S.; Palombarini, Federica; Quaglio, D.; Boffi, A.; Trabuco, M. C.; Baiocco, P.; Fazi, F.; Bonamore, A.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 9:1(2019), p. 11749. [10.1038/s41598-019-48037-z]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1313577
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