CONTEXT: Type 2 diabetes (T2DM) is associated with a higher intestinal expression of the glucose transporters sodium/glucose cotransporter 1 (SGLT-1) and glucose transporter 2 (GLUT-2). It is currently unsettled whether prediabetes conditions characterized by postprandial hyperglycemia, such as impaired glucose tolerance (IGT) and normal glucose tolerance (NGT) with 1-hour postload glucose ≥155 mg/dL (8.6 mmol/L) (NGT-1h-high) are associated with increased expression of these glucose carriers in the intestine. OBJECTIVE: We evaluated whether duodenal abundance of SGLT-1 and GLUT-2 is augmented in subjects with IGT and NGT-1h-high, in comparison with subjects with NGT and 1-hour postload glucose ˂155 mg/dL (NGT-1h-low). DESIGN: Cross-sectional. PATIENTS: A total of 54 individuals underwent an upper gastrointestinal endoscopy. MAIN OUTCOME MEASURES: Duodenal SGLT-1 and GLUT-2 protein and messenger RNA levels were assessed by Western blot and reverse transcription polymerase chain reaction, respectively. RESULTS: Of the 54 subjects examined, 18 had NGT-1h-low, 12 had NGT-1h-high, 12 had IGT, and 12 had T2DM. Duodenal SGLT-1 protein and messenger RNA levels were significantly higher in individuals with NGT-1h-high, IGT, or T2DM in comparison with NGT-1h-low subjects. GLUT-2 abundance was higher in individuals with T2DM in comparison with NGT-1h-low subjects; no substantial increase in GLUT-2 expression was observed in NGT-1h-high or IGT individuals. Univariate correlations showed that duodenal SGLT-1 abundance was positively correlated with 1-hour postload plasma glucose levels (r = 0.44; P = 0.003) but not with fasting or 2-hour postload glucose levels. CONCLUSIONS: Duodenal SGLT-1 expression is increased in individuals with 1-hour postload hyperglycemia or IGT, as well as in subjects with T2DM, and it positively correlates with early postload glucose excursion.

Duodenal sodium/glucose co-transporter 1 expression under fasting conditions is associated with postload hyperglycemia / Fiorentino, Tv; Suraci, E; Arcidiacono, Gp; Cimellaro, A; Mignogna, C; Presta, I; Andreozzi, F; Hribal, Ml; Perticone, F; Donato, G; Luzza, F; Sesti, G. - In: THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM. - ISSN 0021-972X. - 102:11(2017), pp. 3979-3989. [10.1210/jc.2017-00348]

Duodenal sodium/glucose co-transporter 1 expression under fasting conditions is associated with postload hyperglycemia

Sesti G
2017

Abstract

CONTEXT: Type 2 diabetes (T2DM) is associated with a higher intestinal expression of the glucose transporters sodium/glucose cotransporter 1 (SGLT-1) and glucose transporter 2 (GLUT-2). It is currently unsettled whether prediabetes conditions characterized by postprandial hyperglycemia, such as impaired glucose tolerance (IGT) and normal glucose tolerance (NGT) with 1-hour postload glucose ≥155 mg/dL (8.6 mmol/L) (NGT-1h-high) are associated with increased expression of these glucose carriers in the intestine. OBJECTIVE: We evaluated whether duodenal abundance of SGLT-1 and GLUT-2 is augmented in subjects with IGT and NGT-1h-high, in comparison with subjects with NGT and 1-hour postload glucose ˂155 mg/dL (NGT-1h-low). DESIGN: Cross-sectional. PATIENTS: A total of 54 individuals underwent an upper gastrointestinal endoscopy. MAIN OUTCOME MEASURES: Duodenal SGLT-1 and GLUT-2 protein and messenger RNA levels were assessed by Western blot and reverse transcription polymerase chain reaction, respectively. RESULTS: Of the 54 subjects examined, 18 had NGT-1h-low, 12 had NGT-1h-high, 12 had IGT, and 12 had T2DM. Duodenal SGLT-1 protein and messenger RNA levels were significantly higher in individuals with NGT-1h-high, IGT, or T2DM in comparison with NGT-1h-low subjects. GLUT-2 abundance was higher in individuals with T2DM in comparison with NGT-1h-low subjects; no substantial increase in GLUT-2 expression was observed in NGT-1h-high or IGT individuals. Univariate correlations showed that duodenal SGLT-1 abundance was positively correlated with 1-hour postload plasma glucose levels (r = 0.44; P = 0.003) but not with fasting or 2-hour postload glucose levels. CONCLUSIONS: Duodenal SGLT-1 expression is increased in individuals with 1-hour postload hyperglycemia or IGT, as well as in subjects with T2DM, and it positively correlates with early postload glucose excursion.
2017
intestinal glucose-absorption; dual SGLT1/SGLT2 inhibitor; transporter messenger-RNA; type-2 diabetes mellitus; beta-cell function; plasma-glucose;identify subjects; glicemic control; life style
01 Pubblicazione su rivista::01a Articolo in rivista
Duodenal sodium/glucose co-transporter 1 expression under fasting conditions is associated with postload hyperglycemia / Fiorentino, Tv; Suraci, E; Arcidiacono, Gp; Cimellaro, A; Mignogna, C; Presta, I; Andreozzi, F; Hribal, Ml; Perticone, F; Donato, G; Luzza, F; Sesti, G. - In: THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM. - ISSN 0021-972X. - 102:11(2017), pp. 3979-3989. [10.1210/jc.2017-00348]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1312712
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