The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.
ALTERED EXPRESSION OF THE 2 NATURALLY-OCCURRING HUMAN INSULIN-RECEPTOR VARIANTS IN ISOLATED ADIPOCYTES OF NON-INSULIN-DEPENDENT DIABETES-MELLITUS PATIENTS / Sesti, G; Marini, Ma; Tullio, An; Montemurro, A; Borboni, P; Fusco, A; Accili, D; Lauro, R. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 181:3(1991), pp. 1419-1424. [10.1016/0006-291X(91)92097-4]
ALTERED EXPRESSION OF THE 2 NATURALLY-OCCURRING HUMAN INSULIN-RECEPTOR VARIANTS IN ISOLATED ADIPOCYTES OF NON-INSULIN-DEPENDENT DIABETES-MELLITUS PATIENTS
SESTI GPrimo
Writing – Review & Editing
;
1991
Abstract
The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
