Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; thereafter, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treat ment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.
Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells / Borboni, P; Magnaterra, R; Rabini, Ra; Staffolani, R; Porzio, O; Sesti, G; Fusco, A; Mazzanti, L; Lauro, R; Marlier, Lnjl. - In: ACTA DIABETOLOGICA. - ISSN 0940-5429. - 33:2(1996), pp. 154-158.
Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells
Sesti G;
1996
Abstract
Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; thereafter, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treat ment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
