In the present study, we characterize the molecular structure of the GABA(A) receptor in pancreas, islets, alpha and beta cells, and in RIN 1046-38 cells. Using the polymerase chain reaction and specific primers for 11 out of the 15 subunits known so far, that may contribute to the composition of the GABA(A) receptors, we demonstrate that pancreas and its cellular components, as well RIN 1046-38 cells, might contain a GABA(A) receptor resulting from all the possible combinations in a pentameric configuration of the subtypes alpha 1, alpha 2, alpha 3 of the alpha subunit family, beta 1, beta 2, beta 3 subtypes of the beta subunit family, delta subunit and gamma 2 subtype of the gamma subunit family. The presence of the gamma 2 subunit renders the GABA(A) receptors potentially sensitive to allosteric modulators.
MOLECULAR AND CELLULAR CHARACTERIZATION OF THE GABA(A) RECEPTOR IN THE RAT PANCREAS / Borboni, P; Porzio, O; Fusco, A; Sesti, G; Lauro, R; Marlier, Lnjl. - In: MOLECULAR AND CELLULAR ENDOCRINOLOGY. - ISSN 0303-7207. - 103:1-2(1994), pp. 157-163. [10.1016/0303-7207(94)90083-3]
MOLECULAR AND CELLULAR CHARACTERIZATION OF THE GABA(A) RECEPTOR IN THE RAT PANCREAS
SESTI GSupervision
;
1994
Abstract
In the present study, we characterize the molecular structure of the GABA(A) receptor in pancreas, islets, alpha and beta cells, and in RIN 1046-38 cells. Using the polymerase chain reaction and specific primers for 11 out of the 15 subunits known so far, that may contribute to the composition of the GABA(A) receptors, we demonstrate that pancreas and its cellular components, as well RIN 1046-38 cells, might contain a GABA(A) receptor resulting from all the possible combinations in a pentameric configuration of the subtypes alpha 1, alpha 2, alpha 3 of the alpha subunit family, beta 1, beta 2, beta 3 subtypes of the beta subunit family, delta subunit and gamma 2 subtype of the gamma subunit family. The presence of the gamma 2 subunit renders the GABA(A) receptors potentially sensitive to allosteric modulators.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
