Clonal development (clonogenicity) is an inherent property of a subset of postnatal bone marrow (BM) adherent stromal mesenchymal stem cells (MSCs) from which a multipotent progeny develops in culture. Our data suggest that clonogenicity and BM-MSC expansion are two distinct biological events. This hypothesis is based on the following observations: (1) the beginning of clonal growth is a property strictly dependent on serum and independent of the social context, (2) the expansion of individual clone is influenced by events deriving from a social context during initial growth, (3) clonogenic cells grown in a social context in presence of serum can emancipate themselves to generate a secondary different progeny, and (4) the ability of socially generated clones to develop an inherent potential for further growth suggests that quorum sensing may operate in BM-MSC cultures and determine the potential growth of clonal strains.

The potential role of Quorum Sensing in clonal growth and subsequent expansion of bone marrow stromal cell strains in culture / Alimandi, Maurizio; Pierelli, Luca; Pino, Valentina; Gentileschi, Stefano; Sacchetti, Benedetto. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-966X. - 2019:(2019), pp. 1-11. [10.1155/2019/1579102]

The potential role of Quorum Sensing in clonal growth and subsequent expansion of bone marrow stromal cell strains in culture

Alimandi, Maurizio
Co-primo
Writing – Original Draft Preparation
;
Pierelli, Luca
Co-primo
Writing – Review & Editing
;
Sacchetti, Benedetto
Conceptualization
2019

Abstract

Clonal development (clonogenicity) is an inherent property of a subset of postnatal bone marrow (BM) adherent stromal mesenchymal stem cells (MSCs) from which a multipotent progeny develops in culture. Our data suggest that clonogenicity and BM-MSC expansion are two distinct biological events. This hypothesis is based on the following observations: (1) the beginning of clonal growth is a property strictly dependent on serum and independent of the social context, (2) the expansion of individual clone is influenced by events deriving from a social context during initial growth, (3) clonogenic cells grown in a social context in presence of serum can emancipate themselves to generate a secondary different progeny, and (4) the ability of socially generated clones to develop an inherent potential for further growth suggests that quorum sensing may operate in BM-MSC cultures and determine the potential growth of clonal strains.
2019
Bone marrow stroma; mesenchymal stem cells; colony forming unit-fibroblast; post-natal marrow; quorum sensing; clonal growth
01 Pubblicazione su rivista::01a Articolo in rivista
The potential role of Quorum Sensing in clonal growth and subsequent expansion of bone marrow stromal cell strains in culture / Alimandi, Maurizio; Pierelli, Luca; Pino, Valentina; Gentileschi, Stefano; Sacchetti, Benedetto. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-966X. - 2019:(2019), pp. 1-11. [10.1155/2019/1579102]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1310496
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