Topical steroids are effective in Eosinophilic Esophagitis (EoE), but patients often show different tendencies to relapse. We assessed whether gene expression is associated with a sort of "steroid dependency" in EoE children. Methods: Biopsy samples were prospectively collected on EoE children responding to topical steroids. Patients treated with viscous budesonide for 24 weeks were subsequently classified as early (6 months) or late (>6 months) relapsing. RNA was isolated from esophageal biopsies at the time of the relapse and analyzed by NGS for transcriptome profiling. Results: Of 40 patients, 22 patients were considered for mRNA expression profile. Thirteen were included in the early-relapse group, and 9 were in the late-relapse. No significant difference was observed in the two groups for clinical, endoscopic or histological features. Using the mRNA expression profile we performed supervised clustering using the 10 top differentially expressed genes between early and late relapsing patients. The heatmap and PCA show a proper segregation among patients. SERPINB12 is the only gene attaining a significant differential expression between the two groups (FDR < 0.05). Conclusions: Different tendencies to relapse in EoE children responding to topical steroids might be related to altered mRNA expressions. SERPINB12 presented a significantly higher expression.

SERPINB12 as a possible marker of steroid dependency in children with eosinophilic esophagitis: a pilot study / Oliva, Salvatore; Laudadio, Ilaria; Fulci, Valerio; Rossetti, Danilo; Isoldi, Sara; Stronati, Laura; Carissimi, Claudia. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - 52:2(2020), pp. 158-163. [10.1016/j.dld.2019.08.018]

SERPINB12 as a possible marker of steroid dependency in children with eosinophilic esophagitis: a pilot study

Salvatore Oliva
Co-primo
;
Ilaria Laudadio
Co-primo
;
Valerio Fulci;ROSSETTI, DANILO;Sara Isoldi;Laura Stronati
Penultimo
;
Claudia Carissimi
Ultimo
2020

Abstract

Topical steroids are effective in Eosinophilic Esophagitis (EoE), but patients often show different tendencies to relapse. We assessed whether gene expression is associated with a sort of "steroid dependency" in EoE children. Methods: Biopsy samples were prospectively collected on EoE children responding to topical steroids. Patients treated with viscous budesonide for 24 weeks were subsequently classified as early (6 months) or late (>6 months) relapsing. RNA was isolated from esophageal biopsies at the time of the relapse and analyzed by NGS for transcriptome profiling. Results: Of 40 patients, 22 patients were considered for mRNA expression profile. Thirteen were included in the early-relapse group, and 9 were in the late-relapse. No significant difference was observed in the two groups for clinical, endoscopic or histological features. Using the mRNA expression profile we performed supervised clustering using the 10 top differentially expressed genes between early and late relapsing patients. The heatmap and PCA show a proper segregation among patients. SERPINB12 is the only gene attaining a significant differential expression between the two groups (FDR < 0.05). Conclusions: Different tendencies to relapse in EoE children responding to topical steroids might be related to altered mRNA expressions. SERPINB12 presented a significantly higher expression.
2020
eosinophilic esophagitis; SERPINB12; next-generation sequencing; mRNA expression profile; steroid dependency
01 Pubblicazione su rivista::01a Articolo in rivista
SERPINB12 as a possible marker of steroid dependency in children with eosinophilic esophagitis: a pilot study / Oliva, Salvatore; Laudadio, Ilaria; Fulci, Valerio; Rossetti, Danilo; Isoldi, Sara; Stronati, Laura; Carissimi, Claudia. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - 52:2(2020), pp. 158-163. [10.1016/j.dld.2019.08.018]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1306532
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