Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules - 4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG) - were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats. © 2019 Aleksandra Jankovic et al.

Relation of redox and structural alterations of rat skin in the function of chronological aging / Jankovic, A.; Saso, L.; Korac, A.; Korac, B.. - In: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY. - ISSN 1942-0900. - 2019:(2019). [10.1155/2019/2471312]

Relation of redox and structural alterations of rat skin in the function of chronological aging

Saso, L.;
2019

Abstract

Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules - 4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG) - were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats. © 2019 Aleksandra Jankovic et al.
2019
amino acids; biomolecules; enzymes; peptides, glutathione peroxidase; glutathione reductase; methionine sulfoxide reductase; postnatal development; structural alterations; superoxide dismutases; thioredoxin reductase; ultrastructural changes, rats, 4 hydroxynonenal; 8 hydroxyguanine; catalase; collagen; cycline; glutathione peroxidase; glutathione reductase; manganese; methionine sulfoxide reductase a; superoxide dismutase; thioredoxin reductase; 4-hydroxy-2-nonenal; 8-oxo-7-hydrodeoxyguanosine; aldehyde; catalase; deoxyguanosine; glutathione peroxidase; glutathione reductase; msra protein, rat; oxidoreductase; superoxide dismutase; thioredoxin reductase, adult; aged; aging; animal cell; animal experiment; animal model; animal tissue; article; basal lamina; cell proliferation; controlled study; cutaneous parameters; dermoepidermal junction; dna damage; enzyme activity; erythroid precursor cell; extracellular matrix; immunohistochemistry; keratinocyte; lipid peroxidation; male; mitochondrion; nonhuman; oxidation reduction reaction; oxidation reduction state; oxidative stress; postnatal development; protein expression; rat; rough endoplasmic reticulum; sebaceous gland; skin cell; subcutaneous tissue; aging; analogs and derivatives; animal; enzymology; fibroblast; metabolism; oxidation reduction reaction; pathology; skin; time factor; ultrastructure; wistar rat, aging; amino acids; capacity; damage; development; enzymes; mitochondria; peptides, aging; aldehydes; animals; catalase; cell proliferation; collagen; deoxyguanosine; dna damage; fibroblasts; glutathione peroxidase; glutathione reductase; male; oxidation-reduction; oxidoreductases; proliferating cell nuclear antigen; rats, wistar; skin; superoxide dismutase; thioredoxin-disulfide reductase; time factors
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Relation of redox and structural alterations of rat skin in the function of chronological aging / Jankovic, A.; Saso, L.; Korac, A.; Korac, B.. - In: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY. - ISSN 1942-0900. - 2019:(2019). [10.1155/2019/2471312]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1302399
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