Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by -10-30%) and an increase in rotational relaxation times (+10-40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.

In vivo water dynamics in Shewanella oneidensis bacteria at high pressure / Foglia, F.; Hazael, R.; Meersman, F.; Wilding, M. C.; García Sakai, V.; Rogers, S.; Bove, L. E.; Koza, M. M.; Moulin, M.; Haertlein, M.; Forsyth, V. T.; Mcmillan, P. F.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2019). [10.1038/srep18862]

In vivo water dynamics in Shewanella oneidensis bacteria at high pressure

L. E. Bove
;
2019

Abstract

Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by -10-30%) and an increase in rotational relaxation times (+10-40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.
2019
high pressure; bacteria; neutron scattering
01 Pubblicazione su rivista::01a Articolo in rivista
In vivo water dynamics in Shewanella oneidensis bacteria at high pressure / Foglia, F.; Hazael, R.; Meersman, F.; Wilding, M. C.; García Sakai, V.; Rogers, S.; Bove, L. E.; Koza, M. M.; Moulin, M.; Haertlein, M.; Forsyth, V. T.; Mcmillan, P. F.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2019). [10.1038/srep18862]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1291202
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