The object of this study was the recognition of Regions Of Interest(ROIs) in a time series of digital images of two specific laboratory experiments. It concerns the identifcation of objects in a tissue surface by high-resolution and high-speed ad-hoc systems for morphological dynamic image analysis. The protocols and the algorithms implemented are developed to retrieve bio-mechanical properties of two different in vitro systems; the solid lament X-MET (eX-vivo Muscle Engineered Tissue) to measure its reaction to a different frequency stimulation, and a planar system of co-culture of skeletal and cardiac muscle cells, where myotubes and cardiomyocytes coexist, to discriminate the interaction between different cell's type, of its spontaneous pulse. The results of the stimulated X-MET from solid culture system are frequency dependent points of the macroscopic muscular strength and its contractile response. The results for the co-culture planar board measure the correlation of the pulsed movements of the different parts of the tissue.
Analysis of high frame-rate movies by variational methods / Pedone, Massimiliano; Carosio, Silvia; Ruocco, Giancarlo; DEL PRETE, Zaccaria. - In: MATHEMATICS FOR APPLICATIONS. - ISSN 1805-3610. - 8.1:ma.2019.05(2019), pp. 59-77. [10.13164/ma.2019.05]
Analysis of high frame-rate movies by variational methods
MASSIMILIANO PEDONE
;SILVIA CAROSIOInvestigation
;GIANCARLO RUOCCO;ZACCARIA DEL PRETE
2019
Abstract
The object of this study was the recognition of Regions Of Interest(ROIs) in a time series of digital images of two specific laboratory experiments. It concerns the identifcation of objects in a tissue surface by high-resolution and high-speed ad-hoc systems for morphological dynamic image analysis. The protocols and the algorithms implemented are developed to retrieve bio-mechanical properties of two different in vitro systems; the solid lament X-MET (eX-vivo Muscle Engineered Tissue) to measure its reaction to a different frequency stimulation, and a planar system of co-culture of skeletal and cardiac muscle cells, where myotubes and cardiomyocytes coexist, to discriminate the interaction between different cell's type, of its spontaneous pulse. The results of the stimulated X-MET from solid culture system are frequency dependent points of the macroscopic muscular strength and its contractile response. The results for the co-culture planar board measure the correlation of the pulsed movements of the different parts of the tissue.File | Dimensione | Formato | |
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