Background: In a recent multi-centre Italian survey (2003-2004), conducted in 45 laboratories throughout Italy with the aim of monitoring microorganisms responsible for severe infections and their antibiotic resistance, Acinetobacter baumannii was isolated from various wards of 9 hospitals as one of the most frequent pathogens. One hundred and seven clinically significant strains of A. baumannii isolates were included in this study to determine the in vitro activity of tigecycline and comparator agents. Methods: Tests for the susceptibility to antibiotics were performed by the broth microdilution method as recommended by CLSI guidelines. The following antibiotics were tested: aztreonam, piperacillin/tazobactam, ampicillin/sulbactam, ceftazidime, cefepime, imipenem, meropenem tetracycline, doxycycline, tigecycline, gentamicin, amikacin, ciprofloxacin, colistin, and trimethoprim/sulphametoxazole. The PCR assay was used to determine the presence of OXA, VIM, or IMP genes in the carbapenem resistant strains. Results: A. baumannii showed widespread resistance to ceftazidime, ciprofloxacin and aztreonam in more than 90% of the strains; resistance to imipenem and meropenem was 50 and 59% respectively, amikacin and gentamicin were both active against about 30% of the strains and colistin about 99%, with only one strain resistant. By comparison with tetracyclines, tigecycline and doxycycline showed a higher activity. In particular, tigecycline showed a MIC90 value of 2 mg/L and our strains displayed a unimodal distribution of susceptibility being indistinctly active against carbapenem-susceptible and resistant strains, these latter possessed OXA-type variant enzymes. Conclusion: In conclusion, tigecycline had a good activity against the MDR A. baumannii strains while maintaining the same MIC90 of 2 mg/L against the carbapenem-resistant strains. © 2008 Mezzatesta et al; licensee BioMed Central Ltd.

In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy / Mezzatesta M., L; Trovato, G; Gona, F; Nicolosi V., M; Nicolosi, D; Carattoli, A; Fadda, G; Nicolett, G; Stefani, S.. - In: ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS. - ISSN 1476-0711. - 7:(2008). [10.1186/1476-0711-7-4]

In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy

Carattoli A;
2008

Abstract

Background: In a recent multi-centre Italian survey (2003-2004), conducted in 45 laboratories throughout Italy with the aim of monitoring microorganisms responsible for severe infections and their antibiotic resistance, Acinetobacter baumannii was isolated from various wards of 9 hospitals as one of the most frequent pathogens. One hundred and seven clinically significant strains of A. baumannii isolates were included in this study to determine the in vitro activity of tigecycline and comparator agents. Methods: Tests for the susceptibility to antibiotics were performed by the broth microdilution method as recommended by CLSI guidelines. The following antibiotics were tested: aztreonam, piperacillin/tazobactam, ampicillin/sulbactam, ceftazidime, cefepime, imipenem, meropenem tetracycline, doxycycline, tigecycline, gentamicin, amikacin, ciprofloxacin, colistin, and trimethoprim/sulphametoxazole. The PCR assay was used to determine the presence of OXA, VIM, or IMP genes in the carbapenem resistant strains. Results: A. baumannii showed widespread resistance to ceftazidime, ciprofloxacin and aztreonam in more than 90% of the strains; resistance to imipenem and meropenem was 50 and 59% respectively, amikacin and gentamicin were both active against about 30% of the strains and colistin about 99%, with only one strain resistant. By comparison with tetracyclines, tigecycline and doxycycline showed a higher activity. In particular, tigecycline showed a MIC90 value of 2 mg/L and our strains displayed a unimodal distribution of susceptibility being indistinctly active against carbapenem-susceptible and resistant strains, these latter possessed OXA-type variant enzymes. Conclusion: In conclusion, tigecycline had a good activity against the MDR A. baumannii strains while maintaining the same MIC90 of 2 mg/L against the carbapenem-resistant strains. © 2008 Mezzatesta et al; licensee BioMed Central Ltd.
2008
amikacin; aztreonam; beta lactamase; beta lactamase IMP; beta lactamase OXA; beta lactamase VIM; carbapenem; cefepime; ceftazidime; ciprofloxacin; colistin; cotrimoxazole; doxycycline; gentamicin; imipenem; meropenem; piperacillin plus tazobactam; sultamicillin; tetracycline; tetracycline derivative; tigecycline; unclassified drug; antiinfective agent; bacterial DNA; bacterial protein; beta lactamase; carbapenem derivative; drug derivative; minocycline; tigecycline, Acinetobacter baumannii; antibiotic resistance; antibiotic sensitivity; article; bacterial gene; bacterial strain; bacterium isolation; broth dilution; controlled study; in vitro study; Italy; minimum inhibitory concentration; multidrug resistance; nonhuman; polymerase chain reaction; Acinetobacter infection; antibiotic resistance; clinical trial; drug effect; genetics; human; isolation and purification; methodology; microbiological examination; microbiology; multicenter study, Acinetobacter baumannii; Acinetobacter Infections; Anti-Bacterial Agents; Bacterial Proteins; beta-Lactamases; Carbapenems; DNA, Bacterial; Drug Resistance, Bacterial; Humans; Italy; Microbial Sensitivity Tests; Minocycline; Polymerase Chain Reaction
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In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy / Mezzatesta M., L; Trovato, G; Gona, F; Nicolosi V., M; Nicolosi, D; Carattoli, A; Fadda, G; Nicolett, G; Stefani, S.. - In: ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS. - ISSN 1476-0711. - 7:(2008). [10.1186/1476-0711-7-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1285271
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