A fluorescence resonance energy transfer-based assay has been employed to detect mutations at the 86 codon in the DNA gyrase A (gyrA) gene in Campylobacter coli strains. These mutations were associated with ciprofloxacin resistance in strains isolated in Italy in 2000. The mutations in the gyrA gene were detected by real-time PCR amplification followed by hybridization with two fluorescent probes designed with sequences complementary to the wild-type C. coli gyrA gene. Mutation detection was performed by melting peak analysis of the probe-PCR product hybrid performed on a LightCycler (Roche Diagnostic). This gyrA mutation assay allows a rapid and reproducible screening method of ciprofloxacin-resistant C. coli strains. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Use of a LightCycler gyrA mutation assay for identification of ciprofloxacin-resistant Campylobacter coli / Carattoli, A; Dionisi, Am; Luzzi, I.. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 214:1(2002), pp. 87-93. [10.1016/S0378-1097(02)00848-0]
Use of a LightCycler gyrA mutation assay for identification of ciprofloxacin-resistant Campylobacter coli
Carattoli A;
2002
Abstract
A fluorescence resonance energy transfer-based assay has been employed to detect mutations at the 86 codon in the DNA gyrase A (gyrA) gene in Campylobacter coli strains. These mutations were associated with ciprofloxacin resistance in strains isolated in Italy in 2000. The mutations in the gyrA gene were detected by real-time PCR amplification followed by hybridization with two fluorescent probes designed with sequences complementary to the wild-type C. coli gyrA gene. Mutation detection was performed by melting peak analysis of the probe-PCR product hybrid performed on a LightCycler (Roche Diagnostic). This gyrA mutation assay allows a rapid and reproducible screening method of ciprofloxacin-resistant C. coli strains. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
