Objectives: The animal reservoir of plasmid-mediated quinolone resistance (PMQR) and β-lactamases is still controversial and little information is available on the prevalence of these resistance determinants in developing countries. The aim of this study was to identify and characterize PMQR and β-lactamases in a collection of commensal ampicillin-resistant Escherichia coli isolated from healthy chickens and pigs at slaughter, collected in November-December 2006, in Ibadan, Nigeria. Methods: One hundred and sixty-two ampicillin-resistant E. coli were obtained from healthy chickens and pigs at slaughter in Ibadan, Nigeria. Strains were tested for antimicrobial susceptibility by disc diffusion assay. MICs of ciprofloxacin were determined by Etest. Resistance genes were screened by PCR and DNA sequencing. Clonal relatedness of the isolates was determined by enterobacterial repetitive intergenic consensus-PCR. Plasmids were transferred by conjugation and transformation and characterized by PCR-based replicon typing and plasmid multilocus sequence typing. Results: PMQR genes were detected in 18 E. coli strains; 11 of them showed reduced susceptibility to ciprofloxacin. Twelve strains carried qnrS1, three strains carried qnrB19, one strain carried qnrB10 and three strains carried qepA; one strain carried both qepA and qnrB10. All strains carried the blaTEM gene; one strain was positive for the CTX-M-15 extended-spectrum β-lactamase. Conclusions: Our findings suggest that food animals could represent an important reservoir of PMQR in this region of Africa. Previous studies reported high prevalence of qnr genes in clinical isolates from humans in Nigeria, suggesting that the spread of these resistance determinants in this country could be particularly relevant. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria / Fortini, D; Fashae, K; García-Fernández, A; Villa, L; Carattoli, A.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 66:6(2011), pp. 1269-1272. [10.1093/jac/dkr085]

Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria

Carattoli A.
2011

Abstract

Objectives: The animal reservoir of plasmid-mediated quinolone resistance (PMQR) and β-lactamases is still controversial and little information is available on the prevalence of these resistance determinants in developing countries. The aim of this study was to identify and characterize PMQR and β-lactamases in a collection of commensal ampicillin-resistant Escherichia coli isolated from healthy chickens and pigs at slaughter, collected in November-December 2006, in Ibadan, Nigeria. Methods: One hundred and sixty-two ampicillin-resistant E. coli were obtained from healthy chickens and pigs at slaughter in Ibadan, Nigeria. Strains were tested for antimicrobial susceptibility by disc diffusion assay. MICs of ciprofloxacin were determined by Etest. Resistance genes were screened by PCR and DNA sequencing. Clonal relatedness of the isolates was determined by enterobacterial repetitive intergenic consensus-PCR. Plasmids were transferred by conjugation and transformation and characterized by PCR-based replicon typing and plasmid multilocus sequence typing. Results: PMQR genes were detected in 18 E. coli strains; 11 of them showed reduced susceptibility to ciprofloxacin. Twelve strains carried qnrS1, three strains carried qnrB19, one strain carried qnrB10 and three strains carried qepA; one strain carried both qepA and qnrB10. All strains carried the blaTEM gene; one strain was positive for the CTX-M-15 extended-spectrum β-lactamase. Conclusions: Our findings suggest that food animals could represent an important reservoir of PMQR in this region of Africa. Previous studies reported high prevalence of qnr genes in clinical isolates from humans in Nigeria, suggesting that the spread of these resistance determinants in this country could be particularly relevant. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
2011
ampicillin; beta lactamase; beta lactamase CTX M; ciprofloxacin; Escherichia coli protein; extended spectrum beta lactamase; protein oqxAB; protein qepA; protein qnrA; protein qnrB; protein qnrC; protein qnrD; protein qnrS; protein qnrS1; quinoline derived antiinfective agent; unclassified drug, antibiotic resistance; antibiotic sensitivity; article; bacterial gene; bacterial strain; bacterium isolate; bacterium isolation; biotransformation; chicken; commensal Escherichia coli; conjugation; controlled study; disk diffusion; DNA sequence; gene identification; minimum inhibitory concentration; multilocus sequence typing; Nigeria; nonhuman; nucleotide sequence; plasmid; plasmid mediated quinolone resistance; polymerase chain reaction; protein analysis; replicon; Salmonella; Salmonella enterica; swine, Animals; Anti-Bacterial Agents; Bacterial Typing Techniques; beta-Lactamases; beta-Lactams; Carrier State; Chickens; Conjugation, Genetic; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Microbial Sensitivity Tests; Molecular Typing; Multilocus Sequence Typing; Nigeria; Plasmids; Polymerase Chain Reaction; Quinolones; Swine
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Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria / Fortini, D; Fashae, K; García-Fernández, A; Villa, L; Carattoli, A.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 66:6(2011), pp. 1269-1272. [10.1093/jac/dkr085]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1284434
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