The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds. The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed. Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene. This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids.

Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131 / Bonnin, R. A.; Poirel, L; Carattoli, A; Nordmann, P.. - In: PLOS ONE. - ISSN 1932-6203. - 7:4(2012). [10.1371/journal.pone.0034752]

Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131

Carattoli A;
2012

Abstract

The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds. The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed. Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene. This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids.
2012
beta lactamase; beta lactamase NDM 4, E coli; beta lactamase NDM-4, E coli, article; chemistry; DNA sequence; Escherichia coli; genetics; microbial sensitivity test; molecular genetics; multidrug resistance; plasmid, beta-Lactamases; Drug Resistance, Multiple, Bacterial; Escherichia coli; Microbial Sensitivity Tests; Molecular Sequence Data; Plasmids; Sequence Analysis, DNA
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Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131 / Bonnin, R. A.; Poirel, L; Carattoli, A; Nordmann, P.. - In: PLOS ONE. - ISSN 1932-6203. - 7:4(2012). [10.1371/journal.pone.0034752]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1284385
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