Introduction: blaOXA-48, blaNDM-1 and blaCTX-M-3 are clinically relevant resistance genes, frequently associated with the broad-host range plasmids of the IncL/M group. The L and M plasmids belong to two compatible groups, which were incorrectly classified together by molecular methods. In order to understand their evolution, we fully sequenced four IncL/M plasmids, including the reference plasmids R471 and R69, the recently described blaOXA-48-carrying plasmid pKPN-El.Nr7 from a Klebsiella pneumoniae isolated in Bern (Switzerland), and the blaSHV-5 carrying plasmid p202c from a Salmonella enterica from Tirana (Albania). Methods: Sequencing was performed using 454 Junior Genome Sequencer (Roche). Annotation was performed using Sequin and Artemis software. Plasmid sequences were compared with 13 fully sequenced plasmids belonging to the IncL/M group available in GenBank. Results: Comparative analysis of plasmid genomes revealed two distinct genetic lineages, each containing one of the R471 (IncL) and R69 (IncM) reference plasmids. Conjugation experiments demonstrated that plasmids representative of the IncL and IncM groups were compatible with each other. The IncL group is constituted by the blaOXA-48-carrying plasmids and R471. The IncM group contains two sub-types of plasmids named IncM1 and IncM2 that are each incompatible. Conclusion: This work re-defines the structure of the IncL and IncM families and ascribes a definitive designation to the fully sequenced IncL/M plasmids available in GenBank. © 2015 Carattoli et al.

Differentiation of IncL and IncM plasmids associated with the spread of clinically relevant antimicrobial resistance / Carattoli, A; Seiffert S., N; Schwendener, S; Perreten, V; Endimiani, A. - In: PLOS ONE. - ISSN 1932-6203. - 10:5(2015). [10.1371/journal.pone.0123063]

Differentiation of IncL and IncM plasmids associated with the spread of clinically relevant antimicrobial resistance

Carattoli A
;
2015

Abstract

Introduction: blaOXA-48, blaNDM-1 and blaCTX-M-3 are clinically relevant resistance genes, frequently associated with the broad-host range plasmids of the IncL/M group. The L and M plasmids belong to two compatible groups, which were incorrectly classified together by molecular methods. In order to understand their evolution, we fully sequenced four IncL/M plasmids, including the reference plasmids R471 and R69, the recently described blaOXA-48-carrying plasmid pKPN-El.Nr7 from a Klebsiella pneumoniae isolated in Bern (Switzerland), and the blaSHV-5 carrying plasmid p202c from a Salmonella enterica from Tirana (Albania). Methods: Sequencing was performed using 454 Junior Genome Sequencer (Roche). Annotation was performed using Sequin and Artemis software. Plasmid sequences were compared with 13 fully sequenced plasmids belonging to the IncL/M group available in GenBank. Results: Comparative analysis of plasmid genomes revealed two distinct genetic lineages, each containing one of the R471 (IncL) and R69 (IncM) reference plasmids. Conjugation experiments demonstrated that plasmids representative of the IncL and IncM groups were compatible with each other. The IncL group is constituted by the blaOXA-48-carrying plasmids and R471. The IncM group contains two sub-types of plasmids named IncM1 and IncM2 that are each incompatible. Conclusion: This work re-defines the structure of the IncL and IncM families and ascribes a definitive designation to the fully sequenced IncL/M plasmids available in GenBank. © 2015 Carattoli et al.
2015
Antiinfective agent; antiinfective agent, Albania; antibiotic resistance; Article; bacterial gene; bacterial genome; bacterial transmission; bacterium isolate; blaOXA 48 gene; blaSHV 5 gene; controlled study; Escherichia coli; gene sequence; genetic variability; Klebsiella pneumoniae; nonhuman; nucleotide sequence; nucleotide sequence; phylogeny; plasmid; plasmid IncL; plasmid IncM; Salmonella enterica; Salmonella enterica serovar Paratyphi A; Salmonella enterica serovar Typhimurium; sequence alignment; sequence analysis; sequence homology; Serratia marcescens; Switzerland; antibiotic resistance; DNA sequence; drug effects; genetics; molecular genetics; plasmid; polymerase chain reaction; restriction mapping, Klebsiella pneumoniae; Salmonella enterica, Anti-Infective Agents; Base Sequence; Drug Resistance, Bacterial; Genome, Bacterial; Molecular Sequence Data; Phylogeny; Plasmids; Polymerase Chain Reaction; Restriction Mapping; Sequence Analysis, DNA
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Differentiation of IncL and IncM plasmids associated with the spread of clinically relevant antimicrobial resistance / Carattoli, A; Seiffert S., N; Schwendener, S; Perreten, V; Endimiani, A. - In: PLOS ONE. - ISSN 1932-6203. - 10:5(2015). [10.1371/journal.pone.0123063]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1284295
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