By means of functional genomics analysis, we recently described the mRNA expression profiles of various genes involved in the neuroinflammatory response in the brains of subjects with late-onset Alzheimer Disease (LOAD). Some of these genes, namely interleukin (IL)-1b and IL-6, showed distinct expression profiles with peak expression during the first stages of the disease and control-like levels at later stages. IL-1b and IL-6 genes are modulated by DNA methylation in different chronic and degenerative diseases; it is also well known that LOAD may have an epigenetic basis. Indeed, we and others have previously reported gene-specific DNA methylation alterations in LOAD and in related animal models. Based on these data, we studied the DNA methylation profiles, at single cytosine resolution, of IL-1b and IL-6 5'-flanking region by bisulphite modification in the cortex of healthy controls and LOAD patients at 2 different disease stages: Braak I-II/A and Braak V-VI/C. Our analysis provides evidence that neuroinflammation in LOAD is associated with (and possibly mediated by) epigenetic modifications.

DNA methylation profiles of selected pro-inflammatory cytokines in Alzheimer disease / Nicolia, V.; Cavallaro, R. A.; Lopez-Gonzalez, I.; Maccarrone, M.; Scarpa, S.; Ferrer, I.; Fuso, A.. - In: JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY. - ISSN 0022-3069. - 76:1(2017), pp. 27-31. [10.1093/jnen/nlw099]

DNA methylation profiles of selected pro-inflammatory cytokines in Alzheimer disease

Nicolia V.;Cavallaro R. A.;Scarpa S.;Fuso A.
2017

Abstract

By means of functional genomics analysis, we recently described the mRNA expression profiles of various genes involved in the neuroinflammatory response in the brains of subjects with late-onset Alzheimer Disease (LOAD). Some of these genes, namely interleukin (IL)-1b and IL-6, showed distinct expression profiles with peak expression during the first stages of the disease and control-like levels at later stages. IL-1b and IL-6 genes are modulated by DNA methylation in different chronic and degenerative diseases; it is also well known that LOAD may have an epigenetic basis. Indeed, we and others have previously reported gene-specific DNA methylation alterations in LOAD and in related animal models. Based on these data, we studied the DNA methylation profiles, at single cytosine resolution, of IL-1b and IL-6 5'-flanking region by bisulphite modification in the cortex of healthy controls and LOAD patients at 2 different disease stages: Braak I-II/A and Braak V-VI/C. Our analysis provides evidence that neuroinflammation in LOAD is associated with (and possibly mediated by) epigenetic modifications.
2017
Alzheimer disease; cytokines; DNA methylation; epigenetics; neuroinflammation; non-CpG methylation; aged; aged, 80 and over; Alzheimer disease; cytokines; DNA methylation; female; frontal lobe; gene expression profiling; humans; inflammation mediators; male; middle aged; promoter regions; genetic
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DNA methylation profiles of selected pro-inflammatory cytokines in Alzheimer disease / Nicolia, V.; Cavallaro, R. A.; Lopez-Gonzalez, I.; Maccarrone, M.; Scarpa, S.; Ferrer, I.; Fuso, A.. - In: JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY. - ISSN 0022-3069. - 76:1(2017), pp. 27-31. [10.1093/jnen/nlw099]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1283638
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