SUMMARY: Here we introduce ccSOL omics, a webserver for large-scale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis.RESULTS: Using coil/disorder, hydrophobicity, hydrophilicity, β-sheet and α-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing <30% sequence similarity.AVAILABILITY AND IMPLEMENTATION: ccSOL omics can be freely accessed on the web at http://s.tartaglialab.com/page/ccsol_group. Documentation and tutorial are available at http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html.CONTACT: gian.tartaglia@crg.esSUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli / Agostini, Federico; Cirillo, Davide; Livi, Carmen M. aria; Delli Ponti, Riccardo; Tartaglia, Gian G. aetano. - In: BIOINFORMATICS. - ISSN 1367-4811. - 30:20(2014), pp. 2975-2977. [10.1093/bioinformatics/btu420]

ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli

Tartaglia, Gian G. aetano
2014

Abstract

SUMMARY: Here we introduce ccSOL omics, a webserver for large-scale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis.RESULTS: Using coil/disorder, hydrophobicity, hydrophilicity, β-sheet and α-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing <30% sequence similarity.AVAILABILITY AND IMPLEMENTATION: ccSOL omics can be freely accessed on the web at http://s.tartaglialab.com/page/ccsol_group. Documentation and tutorial are available at http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html.CONTACT: gian.tartaglia@crg.esSUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
2014
Algorithms; escherichia coli; escherichia coli proteins; gene expression; hydrophobic and hydrophilic interactions; protein structure, secondary; proteomics; solubility; gene expression regulation, bacterial; internet; statistics and probability; Biochemistry; Molecular Biology; Computer Science Applications1707 Computer Vision and Pattern Recognition; Computational Theory and Mathematics; Computational Mathematics
01 Pubblicazione su rivista::01a Articolo in rivista
ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli / Agostini, Federico; Cirillo, Davide; Livi, Carmen M. aria; Delli Ponti, Riccardo; Tartaglia, Gian G. aetano. - In: BIOINFORMATICS. - ISSN 1367-4811. - 30:20(2014), pp. 2975-2977. [10.1093/bioinformatics/btu420]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1279503
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