Stra8 (stimulated by retinoic acid 8) encodes a protein crucial for mammalian germ cells entering into premeiotic stages. Here, to elucidate the still unknown STRA8 molecular functions, we studied the cellular localization of the protein in several cell types, including premeiotic mouse germ cells and stem cell lines. We reported distinct STRA8 localization in germ and stem cell types and a heterogeneous protein distribution in the cytoplasm and nucleus of such cells suggesting that the protein can shuttle between these two compartments. Moreover, we identified specific protein motifs determining its nuclear import/export. Furthermore, we demonstrated that in transfected cell lines the nuclear import of STRA8 is an active process depending on an N-terminal basic nuclear localization signal. Moreover, its nuclear export is mainly mediated by the Exportin1 (XPO1) recognition of a nuclear export signal. Significantly, we also demonstrated that STRA8 associates with DNA and possesses transcriptional activity. These observations strongly suggest that STRA8 can exert important functions in the nucleus rather than in the cytoplasm as believed previously, likely depending on the cell type and regulated by its nuclear-cytoplasmic shuttling.

STRA8 Shuttles between Nucleus and Cytoplasm and Displays Transcriptional Activity / Tedesco, Marianna; La Sala, Gina; Barbagallo, Federica; De Felici, Massimo; Farini, Donatella. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 284:51(2009), pp. 35781-35793. [10.1074/jbc.M109.056481]

STRA8 Shuttles between Nucleus and Cytoplasm and Displays Transcriptional Activity

Barbagallo, Federica
Membro del Collaboration Group
;
2009

Abstract

Stra8 (stimulated by retinoic acid 8) encodes a protein crucial for mammalian germ cells entering into premeiotic stages. Here, to elucidate the still unknown STRA8 molecular functions, we studied the cellular localization of the protein in several cell types, including premeiotic mouse germ cells and stem cell lines. We reported distinct STRA8 localization in germ and stem cell types and a heterogeneous protein distribution in the cytoplasm and nucleus of such cells suggesting that the protein can shuttle between these two compartments. Moreover, we identified specific protein motifs determining its nuclear import/export. Furthermore, we demonstrated that in transfected cell lines the nuclear import of STRA8 is an active process depending on an N-terminal basic nuclear localization signal. Moreover, its nuclear export is mainly mediated by the Exportin1 (XPO1) recognition of a nuclear export signal. Significantly, we also demonstrated that STRA8 associates with DNA and possesses transcriptional activity. These observations strongly suggest that STRA8 can exert important functions in the nucleus rather than in the cytoplasm as believed previously, likely depending on the cell type and regulated by its nuclear-cytoplasmic shuttling.
2009
germ cells; shuttling; trascriptional activity
01 Pubblicazione su rivista::01a Articolo in rivista
STRA8 Shuttles between Nucleus and Cytoplasm and Displays Transcriptional Activity / Tedesco, Marianna; La Sala, Gina; Barbagallo, Federica; De Felici, Massimo; Farini, Donatella. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 284:51(2009), pp. 35781-35793. [10.1074/jbc.M109.056481]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1279281
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