Atherosclerosis is a chronic inflammatory disorder of the large arteries and represents the primary cause of heart disease and stroke. The exact cause of atherosclerosis is not known. A variety of studies show that autophagy deficiency may be pro-atherogenic and the role of autophagy in smooth muscle cells, macrophages and endothelial cells has been investigated. However, to date no studies addressed the effect of autophagy on lymphocyte subsets playing a role in plaque formation and development. The present project aims to better clarify the role played by autophagy in lymphocytes homeostasis in human atherosclerotic plaques. We characterized lymphocyte populations in different types of lesion by using flow cytometry. In particular, we detected OX40 as marker for conventional T cells promoting division and survival of effector and memory populations and pS6, a marker for an active mTOR pathway and autophagy detection. The understanding of the role of autophagy as a further mechanism underlying lymphocytes stability may open new therapeutic avenues for atherosclerosis.
Evaluation of autophagy in lymphocyte populations during atherosclerotic plaque progression with flow cytometry / Mandatori, Sara; Pacella, Ilenia; Piconese, Silvia; Starace, Donatella; Caprio, Massimiliano; Vitiello, Laura; Armani, Andrea; Marzolla, Vincenzo; Mammi, Caterina; Padula, Fabrizio; Ziparo, Elio; Cecconi, Francesco; Filippini, Antonio. - 28:n. 1 aprile 2019(2019), pp. 42-42. (Intervento presentato al convegno XXXVII National Conference of the Italian Society of Cytometry GIC tenutosi a Paestum).
Evaluation of autophagy in lymphocyte populations during atherosclerotic plaque progression with flow cytometry
Sara MandatoriPrimo
;Ilenia PacellaSecondo
;Silvia Piconese;Donatella Starace;Fabrizio Padula;Elio Ziparo;Antonio FilippiniUltimo
2019
Abstract
Atherosclerosis is a chronic inflammatory disorder of the large arteries and represents the primary cause of heart disease and stroke. The exact cause of atherosclerosis is not known. A variety of studies show that autophagy deficiency may be pro-atherogenic and the role of autophagy in smooth muscle cells, macrophages and endothelial cells has been investigated. However, to date no studies addressed the effect of autophagy on lymphocyte subsets playing a role in plaque formation and development. The present project aims to better clarify the role played by autophagy in lymphocytes homeostasis in human atherosclerotic plaques. We characterized lymphocyte populations in different types of lesion by using flow cytometry. In particular, we detected OX40 as marker for conventional T cells promoting division and survival of effector and memory populations and pS6, a marker for an active mTOR pathway and autophagy detection. The understanding of the role of autophagy as a further mechanism underlying lymphocytes stability may open new therapeutic avenues for atherosclerosis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.