OBJECTIVE: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. METHODS: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. RESULTS: 145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (16.9%) were included in 13 LMTCs, 111 (5.9%) in 20 MMTCs and 236 (12.5%) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7% vs 30.9%, p<0.001), male (82.9% vs 62.3%, p<0.001) and men who have sex with men (MSM) (43.5% vs 14.5%, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR): 109/L: 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. CONCLUSIONS: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.

Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy / Fabeni, Lavinia; Alteri, Claudia; Berno, Giulia; Scutari, Rossana; Orchi, Nicoletta; De Carli, Gabriella; Bertoli, Ada; Carioti, Luca; Gori, Caterina; Forbici, Federica; Salpini, Romina; Vergori, Alessandra; Gagliardini, Roberta; Cicalini, Stefania; Mondi, Annalisa; Pinnetti, Carmela; Mazzuti, Laura; Turriziani, Ombretta; Colafigli, Manuela; Borghi, Vanni; Montella, Francesco; Pennica, Alfredo; Lichtner, Miriam; Girardi, Enrico; Andreoni, Massimo; Mussini, Cristina; Antinori, Andrea; Ceccherini-Silberstein, Francesca; Perno, Carlo Federico; Santoro, Maria Mercedes. - In: SEXUALLY TRANSMITTED INFECTIONS. - ISSN 1368-4973. - (2019), p. sextrans-2019-054017. [10.1136/sextrans-2019-054017]

Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy

Cicalini, Stefania;Mazzuti, Laura;Turriziani, Ombretta;Montella, Francesco;Pennica, Alfredo;Lichtner, Miriam;Andreoni, Massimo;
2019

Abstract

OBJECTIVE: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. METHODS: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. RESULTS: 145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (16.9%) were included in 13 LMTCs, 111 (5.9%) in 20 MMTCs and 236 (12.5%) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7% vs 30.9%, p<0.001), male (82.9% vs 62.3%, p<0.001) and men who have sex with men (MSM) (43.5% vs 14.5%, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR): 109/L: 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. CONCLUSIONS: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.
2019
HIV; molecular epidemiology; transmission dynamics; transmission networks; virology HIV
01 Pubblicazione su rivista::01a Articolo in rivista
Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy / Fabeni, Lavinia; Alteri, Claudia; Berno, Giulia; Scutari, Rossana; Orchi, Nicoletta; De Carli, Gabriella; Bertoli, Ada; Carioti, Luca; Gori, Caterina; Forbici, Federica; Salpini, Romina; Vergori, Alessandra; Gagliardini, Roberta; Cicalini, Stefania; Mondi, Annalisa; Pinnetti, Carmela; Mazzuti, Laura; Turriziani, Ombretta; Colafigli, Manuela; Borghi, Vanni; Montella, Francesco; Pennica, Alfredo; Lichtner, Miriam; Girardi, Enrico; Andreoni, Massimo; Mussini, Cristina; Antinori, Andrea; Ceccherini-Silberstein, Francesca; Perno, Carlo Federico; Santoro, Maria Mercedes. - In: SEXUALLY TRANSMITTED INFECTIONS. - ISSN 1368-4973. - (2019), p. sextrans-2019-054017. [10.1136/sextrans-2019-054017]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1275632
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