Doxorubicin (DOX) is commonly used to treat several tumor types, but its severe side effects, primarily cardiotoxicity, represent a major limitation for its use in clinical settings. In this study we developed and characterized biodegradable and stable poly(D,L-lactic-co-glycolic) acid (PLGA) submicrocarriers employing an osmosis-based patented methodology, which allowed to optimize the drug loading efficiency up to 99%. Proceeding from this, we evaluated on MCF-7, a human breast cancer cell line, the ability of PLGA to promote the internalization of DOX and to improve its cytotoxicity in vitro. We found that the in vitro uptake efficiency is dramatically increased when DOX is loaded within PLGA colloidal carriers, which adhere to the cell membrane behaving as an efficient drug reservoir. In fact, the particles provide a diffusion-driven, sustained release of DOX across the cell membrane, resulting in high drug concentration. Accordingly, the cytotoxic analysis clearly showed that DOX-loaded PLGA exhibit a lower 50% inhibitory concentration than free DOX. The decay time of cell viability was successfully compared with DOX diffusion time constant from PLGA. The overall in vitro results highlight the potential of DOX-loaded PLGA particles to be employed as vectors with improved antitumor efficacy.

PLGA based particles as “drug reservoir” for antitumor drug delivery. Characterization and cytotoxicity studies / Chronopoulou, Laura; Domenici, Fabio; Giantulli, Sabrina; Brasili, Francesco; D'Errico, Chiara; Tsaouli, Georgia; Tortorella, Elisabetta; Bordi, Federico; Morrone, Stefania; Palocci, Cleofe; Silvestri, Ida. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - 180:(2019), pp. 495-502. [10.1016/j.colsurfb.2019.05.006]

PLGA based particles as “drug reservoir” for antitumor drug delivery. Characterization and cytotoxicity studies

Laura Chronopoulou
Co-primo
;
Fabio Domenici
Co-primo
;
Sabrina Giantulli;Francesco Brasili;Chiara D’Errico;Georgia Tsaouli;Elisabetta Tortorella;Federico Bordi;Stefania Morrone;Cleofe Palocci;Ida Silvestri
2019

Abstract

Doxorubicin (DOX) is commonly used to treat several tumor types, but its severe side effects, primarily cardiotoxicity, represent a major limitation for its use in clinical settings. In this study we developed and characterized biodegradable and stable poly(D,L-lactic-co-glycolic) acid (PLGA) submicrocarriers employing an osmosis-based patented methodology, which allowed to optimize the drug loading efficiency up to 99%. Proceeding from this, we evaluated on MCF-7, a human breast cancer cell line, the ability of PLGA to promote the internalization of DOX and to improve its cytotoxicity in vitro. We found that the in vitro uptake efficiency is dramatically increased when DOX is loaded within PLGA colloidal carriers, which adhere to the cell membrane behaving as an efficient drug reservoir. In fact, the particles provide a diffusion-driven, sustained release of DOX across the cell membrane, resulting in high drug concentration. Accordingly, the cytotoxic analysis clearly showed that DOX-loaded PLGA exhibit a lower 50% inhibitory concentration than free DOX. The decay time of cell viability was successfully compared with DOX diffusion time constant from PLGA. The overall in vitro results highlight the potential of DOX-loaded PLGA particles to be employed as vectors with improved antitumor efficacy.
2019
drug delivery; anticancer; Doxorubicin; PLGA carriers; internalization; burst release
01 Pubblicazione su rivista::01a Articolo in rivista
PLGA based particles as “drug reservoir” for antitumor drug delivery. Characterization and cytotoxicity studies / Chronopoulou, Laura; Domenici, Fabio; Giantulli, Sabrina; Brasili, Francesco; D'Errico, Chiara; Tsaouli, Georgia; Tortorella, Elisabetta; Bordi, Federico; Morrone, Stefania; Palocci, Cleofe; Silvestri, Ida. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - 180:(2019), pp. 495-502. [10.1016/j.colsurfb.2019.05.006]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1274339
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