ABSTRACT In the female genital ecosystem, the complex interplay between the host immune system and the resident microflora protects against urogenital pathogens, like Chlamydia trachomatis. C. trachomatis is responsible for urethritis and cervicitis; however, most chlamydial infections are asymptomatic and, thus, not treated, potentially leading to severe reproductive sequelae. Here we investigated the interaction between the levels of selected immune mediators and the community state types of the cervical microbiota in C. trachomatis-infected women. Cervical samples from 42 C. trachomatis-positive women and 103 matched healthy controls were analyzed through the metagenomic analysis of the hypervariable region v4 of the 16S rRNA gene and the determination of lactoferrin, interleukin (IL) 1- alpha , IL-6, interferon-alpha IFN- beta, and IFN-gamma by ELISA. Overall, C. trachomatis infection was significantly associated with a microbiota dominated by anaerobic bacteria (P = 0.000002). In addition, a network of Gardnerella vaginalis, Prevotella amnii, Prevotella buccalis, Prevotella timonensis, Aerococcus christensenii, and Variovorax guangxiensis has been identified as a potential biomarker of C. trachomatis infection through multiple statistical approaches. Again, chlamydial infection was significantly correlated with an increased production of lactoferrin, IL-6, IL-1 alpha, IFN-alpha, and IFN-Beta (P < 0.05), whereas very low levels of IFN-gamma were observed in C. trachomatis-infected women, levels similar to those detected in healthy women. Our findings show a distinctive signature of C. trachomatis genital infection, characterized by a specific bacterial network, constituted by anaerobes, as well as by increased levels of lactoferrin and proinflammatory cytokines (IL-1 alpha, IL-6, IFN-alpha, and IFN-beta), accompanied by low levels of IFN-gamma.

Selected immunological mediators and cervical microbial signatures in women with chlamydia trachomatis infection / Filardo, S; Di Pietro, M; Tranquilli, G; Latino, Ma; Recine, N; Porpora, Mg; Sessa, R. - In: MSYSTEMS. - ISSN 2379-5077. - 4:4(2019), pp. 1-14. [10.1128/mSystems.00094-19]

Selected immunological mediators and cervical microbial signatures in women with chlamydia trachomatis infection

Filardo S;Di Pietro M;Tranquilli G;Recine N;Porpora MG;Sessa R
2019

Abstract

ABSTRACT In the female genital ecosystem, the complex interplay between the host immune system and the resident microflora protects against urogenital pathogens, like Chlamydia trachomatis. C. trachomatis is responsible for urethritis and cervicitis; however, most chlamydial infections are asymptomatic and, thus, not treated, potentially leading to severe reproductive sequelae. Here we investigated the interaction between the levels of selected immune mediators and the community state types of the cervical microbiota in C. trachomatis-infected women. Cervical samples from 42 C. trachomatis-positive women and 103 matched healthy controls were analyzed through the metagenomic analysis of the hypervariable region v4 of the 16S rRNA gene and the determination of lactoferrin, interleukin (IL) 1- alpha , IL-6, interferon-alpha IFN- beta, and IFN-gamma by ELISA. Overall, C. trachomatis infection was significantly associated with a microbiota dominated by anaerobic bacteria (P = 0.000002). In addition, a network of Gardnerella vaginalis, Prevotella amnii, Prevotella buccalis, Prevotella timonensis, Aerococcus christensenii, and Variovorax guangxiensis has been identified as a potential biomarker of C. trachomatis infection through multiple statistical approaches. Again, chlamydial infection was significantly correlated with an increased production of lactoferrin, IL-6, IL-1 alpha, IFN-alpha, and IFN-Beta (P < 0.05), whereas very low levels of IFN-gamma were observed in C. trachomatis-infected women, levels similar to those detected in healthy women. Our findings show a distinctive signature of C. trachomatis genital infection, characterized by a specific bacterial network, constituted by anaerobes, as well as by increased levels of lactoferrin and proinflammatory cytokines (IL-1 alpha, IL-6, IFN-alpha, and IFN-beta), accompanied by low levels of IFN-gamma.
2019
16S rRNA gene; cervical microbiota; chlamydia trachomatis; cytokines; lactoferrin
01 Pubblicazione su rivista::01a Articolo in rivista
Selected immunological mediators and cervical microbial signatures in women with chlamydia trachomatis infection / Filardo, S; Di Pietro, M; Tranquilli, G; Latino, Ma; Recine, N; Porpora, Mg; Sessa, R. - In: MSYSTEMS. - ISSN 2379-5077. - 4:4(2019), pp. 1-14. [10.1128/mSystems.00094-19]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1273931
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