In Saccharomyces cerevisiae the Lysine-acetyltransferase Gcn5 (KAT2) is part of the SAGA complex and is responsible for histone acetylation widely or at specific lysines. In this paper we report that GCN5 deletion differently affects the growth of two strains. The defective mitochondrial phenotype is related to a marked decrease in mtDNA content, which also involves the deletion of specific regions of the molecule. We also show that in wild-type mitochondria the Gcn5 protein is present in the mitoplasts, suggesting a new mitochondrial function independent from the SAGA complex and possibly a new function for this protein connecting epigenetics and metabolism.

Gcn5 histone acetyltransferase is present in the mitoplasts / Montanari, Arianna; Leo, Manuela; DE LUCA, Veronica; Filetici, Patrizia; Francisci, Silvia. - In: BIOLOGY OPEN. - ISSN 2046-6390. - 8:2(2019). [10.1242/bio.041244]

Gcn5 histone acetyltransferase is present in the mitoplasts

Montanari, Arianna
;
Leo, Manuela;DE LUCA, Veronica;Filetici, Patrizia;Francisci, Silvia
2019

Abstract

In Saccharomyces cerevisiae the Lysine-acetyltransferase Gcn5 (KAT2) is part of the SAGA complex and is responsible for histone acetylation widely or at specific lysines. In this paper we report that GCN5 deletion differently affects the growth of two strains. The defective mitochondrial phenotype is related to a marked decrease in mtDNA content, which also involves the deletion of specific regions of the molecule. We also show that in wild-type mitochondria the Gcn5 protein is present in the mitoplasts, suggesting a new mitochondrial function independent from the SAGA complex and possibly a new function for this protein connecting epigenetics and metabolism.
2019
lysine-acetyltransferase Gcn5; mitochondria; mitochondrial DNA; respiration; yeast; biochemistry, genetics and molecular biology (all); agricultural and biological sciences (all)
01 Pubblicazione su rivista::01a Articolo in rivista
Gcn5 histone acetyltransferase is present in the mitoplasts / Montanari, Arianna; Leo, Manuela; DE LUCA, Veronica; Filetici, Patrizia; Francisci, Silvia. - In: BIOLOGY OPEN. - ISSN 2046-6390. - 8:2(2019). [10.1242/bio.041244]
File allegati a questo prodotto
File Dimensione Formato  
Montanari_Gcn5_2019.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 4.46 MB
Formato Adobe PDF
4.46 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1263061
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 12
social impact