The proteasomal lid subunit Rpn11 is essential for maintaining a correct cell cycle and mitochondrial morphology in Saccharomyces cerevisiae. In this paper, we show that the rpn11-m1 mutant has a peculiar cell cycle defect reminiscent of mutants defective in the FEAR pathway that delay the release of the Cdc14 protein phosphatase from the nucleolus. We analyzed the rpn11-m1 phenotypes and found that overexpression of Cdc14 suppresses all the rpn11-m1 defects, including the mitochondrial ones. Suppression by Cdc14 of the rpn11-m1 mitochondrial morphology defect reveals an uncharacterized connection between mitochondrial and cell cycle events. Interestingly, the overexpression of Cdc14 also partially restores the tubular network in an delta mmm2 strain, which lacks a mitochondrial protein belonging to the complex necessary to anchor the mitochondrion to the actin cytoskeleton. Altogether our findings indicate, for the first time, a cross-talk between the cell cycle and mitochondrial morphology.

Analysis of the rpn11-m1 proteasomal mutant reveals connection between cell cycle and mitochondrial biogenesis / Esposito, Michela; Simonetta, Piatti; Line, Hofmann; Frontali, Laura; Agnes, Delahodde; Rinaldi, Teresa. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 11:1(2011), pp. 60-71. [10.1111/j.1567-1364.2010.00690.x]

Analysis of the rpn11-m1 proteasomal mutant reveals connection between cell cycle and mitochondrial biogenesis

ESPOSITO, MICHELA;FRONTALI, Laura;RINALDI, Teresa
2011

Abstract

The proteasomal lid subunit Rpn11 is essential for maintaining a correct cell cycle and mitochondrial morphology in Saccharomyces cerevisiae. In this paper, we show that the rpn11-m1 mutant has a peculiar cell cycle defect reminiscent of mutants defective in the FEAR pathway that delay the release of the Cdc14 protein phosphatase from the nucleolus. We analyzed the rpn11-m1 phenotypes and found that overexpression of Cdc14 suppresses all the rpn11-m1 defects, including the mitochondrial ones. Suppression by Cdc14 of the rpn11-m1 mitochondrial morphology defect reveals an uncharacterized connection between mitochondrial and cell cycle events. Interestingly, the overexpression of Cdc14 also partially restores the tubular network in an delta mmm2 strain, which lacks a mitochondrial protein belonging to the complex necessary to anchor the mitochondrion to the actin cytoskeleton. Altogether our findings indicate, for the first time, a cross-talk between the cell cycle and mitochondrial morphology.
cdc14; mitochondrial morphology; proteasome; rdna; tubulation apparatus; yeast
01 Pubblicazione su rivista::01a Articolo in rivista
Analysis of the rpn11-m1 proteasomal mutant reveals connection between cell cycle and mitochondrial biogenesis / Esposito, Michela; Simonetta, Piatti; Line, Hofmann; Frontali, Laura; Agnes, Delahodde; Rinaldi, Teresa. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 11:1(2011), pp. 60-71. [10.1111/j.1567-1364.2010.00690.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/126225
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