Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB). Functionalization of the Au‐MWCNTs/polyMB platform with the lactate oxidase (LOX) enzyme (working electrode 1) and with the FAD‐Glucose dehydrogenase (FADGDH) enzyme (working electrode 2) enabled the continuous monitoring of lactate and glucose in the artificial ISF. The lactate biosensor exhibited a high sensitivity (797.4±38.1 μA cm−2 mM−1), a good linear range (10–100 μM) with a detection limit of 3 μM. The performance of the glucose biosensor were also good with a sensitivity of 405.2±24.1 μA cm−2 mM−1, a linear range between 0.05 and 5 mM and a detection limit of 7 μM. The biosensor array was tested to detect the amount of lactate generated after 100 minutes of cycling exercise (12 mM) and of glucose after a normal meal for a healthy patient (10 mM). The results reveal that the new microneedles‐based biosensor array seems to be a promising tool for the development of real‐time wearable devices with a variety of sport medicine and clinical care applications.

Minimally-invasive microneedle-based biosensor array for simultaneous lactate and glucose monitoring in artificial interstitial fluid / Bollella, P.; Sharma, S.; Cass, A. E. G.; Antiochia, R.. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 31:2(2019), pp. 374-382. [10.1002/elan.201800630]

Minimally-invasive microneedle-based biosensor array for simultaneous lactate and glucose monitoring in artificial interstitial fluid

P. Bollella
Primo
;
R. Antiochia
Ultimo
Writing – Review & Editing
2019

Abstract

Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB). Functionalization of the Au‐MWCNTs/polyMB platform with the lactate oxidase (LOX) enzyme (working electrode 1) and with the FAD‐Glucose dehydrogenase (FADGDH) enzyme (working electrode 2) enabled the continuous monitoring of lactate and glucose in the artificial ISF. The lactate biosensor exhibited a high sensitivity (797.4±38.1 μA cm−2 mM−1), a good linear range (10–100 μM) with a detection limit of 3 μM. The performance of the glucose biosensor were also good with a sensitivity of 405.2±24.1 μA cm−2 mM−1, a linear range between 0.05 and 5 mM and a detection limit of 7 μM. The biosensor array was tested to detect the amount of lactate generated after 100 minutes of cycling exercise (12 mM) and of glucose after a normal meal for a healthy patient (10 mM). The results reveal that the new microneedles‐based biosensor array seems to be a promising tool for the development of real‐time wearable devices with a variety of sport medicine and clinical care applications.
2019
microneedles; biosensor; minimally invasive; glucose; lactate
01 Pubblicazione su rivista::01a Articolo in rivista
Minimally-invasive microneedle-based biosensor array for simultaneous lactate and glucose monitoring in artificial interstitial fluid / Bollella, P.; Sharma, S.; Cass, A. E. G.; Antiochia, R.. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 31:2(2019), pp. 374-382. [10.1002/elan.201800630]
File allegati a questo prodotto
File Dimensione Formato  
Bollella_Minimally_2019.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 3.58 MB
Formato Adobe PDF
3.58 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1261835
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 103
  • ???jsp.display-item.citation.isi??? 87
social impact