The fragile histidine triad (FHIT) gene at chromosome 3p14.2 is a tumor suppressor gene that is altered mainly by deletion in a large fraction of human tumors, including breast cancers. To evaluate the potential of FHIT gene therapy in this type of cancer, we have studied the biological effects of adenoviral FHIT transduction (Ad-FHIT) in breast cancer cell lines. The results showed that, after FHIT restoration in BT-549, MDA-MB-436, and HCC1806 cells, they underwent apoptosis by activation of the intrinsic pathway. In all three cell lines infected with Ad-FHIT, we have found activation of caspase-2, which is required for permeabilization of mitochondria, release of cytochrome c, and apoptosis. Furthermore, Fhit overexpression produces alteration in cell cycling properties, as well as reduction of the tumorigenic potential in nude mice.

Restoration of fragile Histidine triad (FHIT) expression induces apoptosis and suppresses tumorigenicity in breast cancer cell lines / C., Sevignani; G. A., Calin; R., Ceasri; M., Sarti; H., Hishii; S., Yendamuri; Vecchione, Andrea; F., Trapasso; C. M., Croce. - In: CANCER RESEARCH. - ISSN 0008-5472. - 63:6(2003), pp. 1183-1187.

Restoration of fragile Histidine triad (FHIT) expression induces apoptosis and suppresses tumorigenicity in breast cancer cell lines

VECCHIONE, ANDREA;
2003

Abstract

The fragile histidine triad (FHIT) gene at chromosome 3p14.2 is a tumor suppressor gene that is altered mainly by deletion in a large fraction of human tumors, including breast cancers. To evaluate the potential of FHIT gene therapy in this type of cancer, we have studied the biological effects of adenoviral FHIT transduction (Ad-FHIT) in breast cancer cell lines. The results showed that, after FHIT restoration in BT-549, MDA-MB-436, and HCC1806 cells, they underwent apoptosis by activation of the intrinsic pathway. In all three cell lines infected with Ad-FHIT, we have found activation of caspase-2, which is required for permeabilization of mitochondria, release of cytochrome c, and apoptosis. Furthermore, Fhit overexpression produces alteration in cell cycling properties, as well as reduction of the tumorigenic potential in nude mice.
2003
01 Pubblicazione su rivista::01a Articolo in rivista
Restoration of fragile Histidine triad (FHIT) expression induces apoptosis and suppresses tumorigenicity in breast cancer cell lines / C., Sevignani; G. A., Calin; R., Ceasri; M., Sarti; H., Hishii; S., Yendamuri; Vecchione, Andrea; F., Trapasso; C. M., Croce. - In: CANCER RESEARCH. - ISSN 0008-5472. - 63:6(2003), pp. 1183-1187.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/125668
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