Introduction: Alpha-synuclein (α-syn) aggregation is the pathological hallmark of Parkinson's Disease (PD). In this study, we measured α-syn total (α-syn total ), oligomeric α-syn (α-syn olig ) and α-syn olig /α-syn total ratio in the saliva of patients affected by PD and in age and sex-matched healthy subjects. We also compared salivary α-syn total measured in PD with those detected in Progressive Supranuclear Palsy (PSP), in order to assess whether salivary α-syn can be used as a biomarker for PD and for the differential diagnosis between PD and PSP. Methods: We studied 100 PD patients, 20 patients affected by PSP and 80 age- and sex-matched healthy subjects. ELISA analysis was performed using two commercial ELISA platforms and a specific ELISA assay for α-syn aggregates. Results: We detected lower α-syn total and higher α-syn olig in PD than in healthy subjects. Conversely in PSP salivary α-syn total concentration was comparable to that measured in healthy subjects. Receiver Operating Characteristic analyses revealed specific cut-off values able to differentiate PD patients from healthy subjects and PSP patients with high sensitivity and specificity. However, there was no significant correlation between clinical and molecular data. Conclusion: Salivary α-syn detection could be a promising and easily accessible biomarker for PD and for the differential diagnosis between PD and PSP.

Salivary alpha-synuclein in the diagnosis of parkinson's disease and progressive supranuclear palsy / Vivacqua, Giorgio; Suppa, Antonio; Mancinelli, Romina; Belvisi, Daniele; Fabbrini, Andrea; Costanzo, Matteo; Formica, Alessandra; Onori, Paolo; Fabbrini, Giovanni; Berardelli, Alfredo. - In: PARKINSONISM & RELATED DISORDERS. - ISSN 1353-8020. - 63:(2019), pp. 143-148. [10.1016/j.parkreldis.2019.02.014]

Salivary alpha-synuclein in the diagnosis of parkinson's disease and progressive supranuclear palsy

Suppa, Antonio;Mancinelli, Romina;Belvisi, Daniele;Fabbrini, Andrea;Costanzo, Matteo;Formica, Alessandra;Onori, Paolo;Fabbrini, Giovanni;Berardelli, Alfredo
2019

Abstract

Introduction: Alpha-synuclein (α-syn) aggregation is the pathological hallmark of Parkinson's Disease (PD). In this study, we measured α-syn total (α-syn total ), oligomeric α-syn (α-syn olig ) and α-syn olig /α-syn total ratio in the saliva of patients affected by PD and in age and sex-matched healthy subjects. We also compared salivary α-syn total measured in PD with those detected in Progressive Supranuclear Palsy (PSP), in order to assess whether salivary α-syn can be used as a biomarker for PD and for the differential diagnosis between PD and PSP. Methods: We studied 100 PD patients, 20 patients affected by PSP and 80 age- and sex-matched healthy subjects. ELISA analysis was performed using two commercial ELISA platforms and a specific ELISA assay for α-syn aggregates. Results: We detected lower α-syn total and higher α-syn olig in PD than in healthy subjects. Conversely in PSP salivary α-syn total concentration was comparable to that measured in healthy subjects. Receiver Operating Characteristic analyses revealed specific cut-off values able to differentiate PD patients from healthy subjects and PSP patients with high sensitivity and specificity. However, there was no significant correlation between clinical and molecular data. Conclusion: Salivary α-syn detection could be a promising and easily accessible biomarker for PD and for the differential diagnosis between PD and PSP.
2019
alpha-synuclein; biomarkers; parkinson's disease; progressive supranuclear palsy; saliva; neurology; geriatrics and gerontology; neurology (clinical)
01 Pubblicazione su rivista::01a Articolo in rivista
Salivary alpha-synuclein in the diagnosis of parkinson's disease and progressive supranuclear palsy / Vivacqua, Giorgio; Suppa, Antonio; Mancinelli, Romina; Belvisi, Daniele; Fabbrini, Andrea; Costanzo, Matteo; Formica, Alessandra; Onori, Paolo; Fabbrini, Giovanni; Berardelli, Alfredo. - In: PARKINSONISM & RELATED DISORDERS. - ISSN 1353-8020. - 63:(2019), pp. 143-148. [10.1016/j.parkreldis.2019.02.014]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1254380
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