In some varieties of citrus, exceptionally low fruit acidity is associated with absence of anthocyanin pigments in leaves and flowers and proanthocyanidins in seeds. Taking advantage of natural variation, Butelli et al. show that this pleiotropic phenotype is the result of mutations in a single gene, Noemi, encoding a bHLH transcription factor. In citrus, the production of anthocyanin pigments requires the activity of the transcriptional activator Ruby. Consequently, loss-of-function mutations in Ruby result in an anthocyaninless phenotype [1]. Several citrus accessions, however, have lost the ability to produce these pigments despite the presence of wild-type Ruby alleles. These specific mutants have captivated the interest of botanists and breeders for centuries because the lack of anthocyanins in young leaves and flowers is also associated with a lack of proanthocyanidins in seeds and, most notably, with an extreme reduction in fruit acidity (involving about a three-unit change in pH). These mutants have been defined collectively as “acidless” [2–4]. We have identified Noemi, which encodes a basic helix-loop-helix (bHLH) transcription factor and which controls these apparently unrelated processes. In accessions of Citron, limetta, sweet lime, lemon, and sweet orange, acidless phenotypes are associated with large deletions or insertions of retrotransposons in the Noemi gene. In two accessions of limetta, a change in the core promoter region of Noemi is associated with reduced expression and increased pH of juice, indicating that Noemi is a major determinant of fruit acidity. The characterization of the Noemi locus in a number of varieties of Citron indicates that one specific mutation is ancient. The presence of this allele in Chinese fingered Citrons and in those used in the Sukkot Jewish ritual [5] illuminates the path of domestication of Citron, the first citrus species to be cultivated in the Mediterranean. This allele has been inherited in Citron-derived hybrids with long histories of cultivation.

Noemi controls production of flavonoid pigments and fruit acidity and Illustrates the domestication routes of modern Citrus varieties / Butelli, Eugenio; Licciardello, Concetta; Ramadugu, Chandrika; Durand-Hulak, Marie; Celant, Alessandra; Reforgiato Recupero, Giuseppe; Froelicher, Yann; Martin, Cathie. - In: CURRENT BIOLOGY. - ISSN 0960-9822. - 29:1(2019), pp. 158-164. [10.1016/j.cub.2018.11.040]

Noemi controls production of flavonoid pigments and fruit acidity and Illustrates the domestication routes of modern Citrus varieties

Celant, Alessandra
Membro del Collaboration Group
;
2019

Abstract

In some varieties of citrus, exceptionally low fruit acidity is associated with absence of anthocyanin pigments in leaves and flowers and proanthocyanidins in seeds. Taking advantage of natural variation, Butelli et al. show that this pleiotropic phenotype is the result of mutations in a single gene, Noemi, encoding a bHLH transcription factor. In citrus, the production of anthocyanin pigments requires the activity of the transcriptional activator Ruby. Consequently, loss-of-function mutations in Ruby result in an anthocyaninless phenotype [1]. Several citrus accessions, however, have lost the ability to produce these pigments despite the presence of wild-type Ruby alleles. These specific mutants have captivated the interest of botanists and breeders for centuries because the lack of anthocyanins in young leaves and flowers is also associated with a lack of proanthocyanidins in seeds and, most notably, with an extreme reduction in fruit acidity (involving about a three-unit change in pH). These mutants have been defined collectively as “acidless” [2–4]. We have identified Noemi, which encodes a basic helix-loop-helix (bHLH) transcription factor and which controls these apparently unrelated processes. In accessions of Citron, limetta, sweet lime, lemon, and sweet orange, acidless phenotypes are associated with large deletions or insertions of retrotransposons in the Noemi gene. In two accessions of limetta, a change in the core promoter region of Noemi is associated with reduced expression and increased pH of juice, indicating that Noemi is a major determinant of fruit acidity. The characterization of the Noemi locus in a number of varieties of Citron indicates that one specific mutation is ancient. The presence of this allele in Chinese fingered Citrons and in those used in the Sukkot Jewish ritual [5] illuminates the path of domestication of Citron, the first citrus species to be cultivated in the Mediterranean. This allele has been inherited in Citron-derived hybrids with long histories of cultivation.
2019
anthocyanins; citrus; domestication; fruit acidity; gene expression
01 Pubblicazione su rivista::01a Articolo in rivista
Noemi controls production of flavonoid pigments and fruit acidity and Illustrates the domestication routes of modern Citrus varieties / Butelli, Eugenio; Licciardello, Concetta; Ramadugu, Chandrika; Durand-Hulak, Marie; Celant, Alessandra; Reforgiato Recupero, Giuseppe; Froelicher, Yann; Martin, Cathie. - In: CURRENT BIOLOGY. - ISSN 0960-9822. - 29:1(2019), pp. 158-164. [10.1016/j.cub.2018.11.040]
File allegati a questo prodotto
File Dimensione Formato  
Butelli_Noemi_2018.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.29 MB
Formato Adobe PDF
2.29 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1253372
Citazioni
  • ???jsp.display-item.citation.pmc??? 23
  • Scopus 87
  • ???jsp.display-item.citation.isi??? 79
social impact