In an attempt to identify new nuclear genes involved in the synthesis and processing of mitochondrial tRNAs, we utilized a multicopy nuclear library to suppress the heat-sensitive phenotype of a Saccharomyces cerevisiae mitochondrial mutant strain. This strain (Ts 932) is defective in the 3¢-end processing of the mitochondrial tRNAAsp transcript. The nuclear genes coding for the mitochondrial elongation factor Tuf M and for the mitochondrial aspartyl-tRNA synthetase have been found to restore the temperature-resistant phenotype and to correct the RNA processing defect. Suppression was effective even when the genes were present on a centromeric plasmid.
Additional copies of the Ef-Tu and Aspartyl-tRNA synthetase genes can compensate a mutation affecting the maturation of the mitochondrial tRNAAsp / Rinaldi, Teresa; Lande, R; BOLOTIN FUKUHARA, M; Frontali, Laura. - In: CURRENT GENETICS. - ISSN 0172-8083. - 31:(1997), pp. 494-496. [10.1007/s002940050235]