Bacterial canker of Actinidia, caused by the bacterium Pseudomonas syringae pv. actinidiae (Psa), is the most serious disease of these plants worldwide. Leaves of three species of Actinidia, namely A. chinensis var. chinensis, A. chinensis var. deliciosa and A. arguta, having different degrees of tolerance to Psa, were analyzed by Nuclear Magnetic Resonance spectroscopy. Aqueous extracts of leaves were studied and several metabolites, classified as organic acids, amino acids, carbohydrates, phenols and other metabolites, were identified by 1D and 2D NMR experiments and quantified. The metabolic profiles of these species were compared through univariate statistical analysis ANOVA and multivariate PCA. Levels of metabolites with known antibacterial activity, such as caffeic and chlorogenic acids, were observed to be higher in the A. arguta samples. Moreover, these metabolites have different Pearson correlation patterns among the three Actinidia species, suggesting a difference at the phenylpropanoid biosynthetic pathway. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.

NMR-based metabolic study of leaves of three species of Actinidia with different degrees of susceptibility to Pseudomonas syringae pv. actinidiae / Sciubba, Fabio; Di Cocco, Maria Enrica; Angori, Giulia; Spagnoli, Mariangela; De Salvador, Flavio Roberto; Engel, Petra; Delfini, Maurizio. - In: NATURAL PRODUCT RESEARCH. - ISSN 1478-6419. - 34:14(2019), pp. 2043-2050. [10.1080/14786419.2019.1574784]

NMR-based metabolic study of leaves of three species of Actinidia with different degrees of susceptibility to Pseudomonas syringae pv. actinidiae

Sciubba, Fabio
;
Di Cocco, Maria Enrica;ANGORI, GIULIA;Delfini, Maurizio
2019

Abstract

Bacterial canker of Actinidia, caused by the bacterium Pseudomonas syringae pv. actinidiae (Psa), is the most serious disease of these plants worldwide. Leaves of three species of Actinidia, namely A. chinensis var. chinensis, A. chinensis var. deliciosa and A. arguta, having different degrees of tolerance to Psa, were analyzed by Nuclear Magnetic Resonance spectroscopy. Aqueous extracts of leaves were studied and several metabolites, classified as organic acids, amino acids, carbohydrates, phenols and other metabolites, were identified by 1D and 2D NMR experiments and quantified. The metabolic profiles of these species were compared through univariate statistical analysis ANOVA and multivariate PCA. Levels of metabolites with known antibacterial activity, such as caffeic and chlorogenic acids, were observed to be higher in the A. arguta samples. Moreover, these metabolites have different Pearson correlation patterns among the three Actinidia species, suggesting a difference at the phenylpropanoid biosynthetic pathway. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
2019
Actinidia arguta; Actinidia chinensis var. chinensis; Actinidia chinensis var. deliciosa; metabolic profile; NMR; Pseudomonas syringae pv. actinidiae
01 Pubblicazione su rivista::01a Articolo in rivista
NMR-based metabolic study of leaves of three species of Actinidia with different degrees of susceptibility to Pseudomonas syringae pv. actinidiae / Sciubba, Fabio; Di Cocco, Maria Enrica; Angori, Giulia; Spagnoli, Mariangela; De Salvador, Flavio Roberto; Engel, Petra; Delfini, Maurizio. - In: NATURAL PRODUCT RESEARCH. - ISSN 1478-6419. - 34:14(2019), pp. 2043-2050. [10.1080/14786419.2019.1574784]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1249544
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