An enzymatic treatment with cell wall degrading enzymes aimed at improving the recovery of lipids from Chlorella sorokiniana was investigated. Six commercial enzyme preparations containing cellulase, pectinase, lysozyme and three different hemicellulases were preliminary tested. The most effective preparations were then used as basic components for the formulation of enzyme cocktails with high cell wall degrading activity. Experiments were carried out according to the mixture design methodology. Analysis of the results revealed the occurrence of both synergistic and antagonistic effects which were, respectively, attributed to the cooperative destructuring of the cell wall and to non-productive competitive adsorption phenomena. A numerical optimization procedure was used to design an optimal enzyme mixture containing β-1,4-xylanase and β-1,4-mannanase in the ratio of 61:39 (% w/w). Pretreatment of C. sorokiniana by this mixture provided a lipid extraction yield of (74.74 ± 1.02)%. For comparison, the extraction yields from the untreated and water-pretreated biomass were, respectively, equal to (36.19 ± 2.33)% and (58.36 ± 1.22)%.

Use of cell wall degrading enzymes to improve the recovery of lipids from Chlorella sorokiniana / Zuorro, Antonio; Malavasi, Veronica; Cao, Giacomo; Lavecchia, Roberto. - In: CHEMICAL ENGINEERING JOURNAL. - ISSN 1385-8947. - 377:(2018). [10.1016/j.cej.2018.11.023]

Use of cell wall degrading enzymes to improve the recovery of lipids from Chlorella sorokiniana

Zuorro, Antonio;CAO, GIACOMO;Lavecchia, Roberto
2018

Abstract

An enzymatic treatment with cell wall degrading enzymes aimed at improving the recovery of lipids from Chlorella sorokiniana was investigated. Six commercial enzyme preparations containing cellulase, pectinase, lysozyme and three different hemicellulases were preliminary tested. The most effective preparations were then used as basic components for the formulation of enzyme cocktails with high cell wall degrading activity. Experiments were carried out according to the mixture design methodology. Analysis of the results revealed the occurrence of both synergistic and antagonistic effects which were, respectively, attributed to the cooperative destructuring of the cell wall and to non-productive competitive adsorption phenomena. A numerical optimization procedure was used to design an optimal enzyme mixture containing β-1,4-xylanase and β-1,4-mannanase in the ratio of 61:39 (% w/w). Pretreatment of C. sorokiniana by this mixture provided a lipid extraction yield of (74.74 ± 1.02)%. For comparison, the extraction yields from the untreated and water-pretreated biomass were, respectively, equal to (36.19 ± 2.33)% and (58.36 ± 1.22)%.
2018
Cell wall degrading enzymes; Chlorella sorokiniana; lipid recovery; microalgae; mixture design; chemistry (all); environmental chemistry; chemical engineering (all); industrial and manufacturing engineering
01 Pubblicazione su rivista::01a Articolo in rivista
Use of cell wall degrading enzymes to improve the recovery of lipids from Chlorella sorokiniana / Zuorro, Antonio; Malavasi, Veronica; Cao, Giacomo; Lavecchia, Roberto. - In: CHEMICAL ENGINEERING JOURNAL. - ISSN 1385-8947. - 377:(2018). [10.1016/j.cej.2018.11.023]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1246589
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