Heavy metal accumulation in the Lichen Evernia prunastri transplanted at urban, rural and industrial sites in Central Italy

The lichen Evernia prunastri has been employed for biomonitoring the atmospheric deposition of heavy metals at urban, rural and industrial sites in Central Italy. Lichen samples have been collected in a control site 1500 m a. s. l. (Parco Nazionale d’Abruzzo, Central Italy) and subsequently transplanted at urban site (Cassino city center), at rural location 7 km away from Cassino (S. Elia Fiumerapido) and at industrial location (Piedimonte S. Germano) surrounding an automobile factory. Once defined the surface of impact relevant to this work, the lichen samples were transplanted at the four cardinal points of each site. Studies of bioaccumulation of Pb, Cd, Cr, Cu and Zn in lichen samples were performed five times at regular intervals between November 2000–December 2001. Microwave digestion of the samples and graphite furnace atomic absorption spectrometry were employed for the heavy metal determinations. Suitable certified reference materials (CRM) were used for validation of the analytical methodology. Results showed the ability of Evernia prunastri to accumulate the heavy metals under study. As expected, the area chosen as control site showed significantly (Friedman test, cluster analysis) lower impact in comparison to the other sites and the rural site showed smaller impact than the urban and the industrial sites.