OBJECTIVE: The influence of sex on gut mucosal T-cell response in HIV-1 infection remains largely unknown. We explored whether the frequencies of interferon-γ and/or IL-17 producing naive, T central memory and T effector memory (TEM) CD4 (Th1, Th17) and CD8 T (Tc1, Tc17) cells measured in gut and peripheral districts differed between female and male HIV-1-infected patients. METHODS: Thirty long-term-treated HIV-1-infected individuals were enrolled. The frequencies of Th1, Th17, Tc1, Tc17-cell subsets (single and double) were evaluated by multiparametric flow cytometry in lamina propria lymphocytes and peripheral blood mononuclear cells (PBMC). RESULTS: A sex-based pattern was recorded in the differences of Th1, Th17, Tc1, Tc17-cell subset (single and double) frequencies between gut and peripheral blood. Female patients had stronger alterations in the gut mucosal T-cell repertoire, especially increased Th1, Th17, and Th1/Th17-cell subset frequencies, compared with the blood district than their male counterparts. Higher naive Tc1, Tc17, Tc1/Tc17, TEM Tc17, and TEM Tc1/Tc17 levels were also recorded in the gut mucosa than in the PBMC of HIV-1-infected women. Males and females also differed in their gut T-cell response, with women being characterized by higher Th1, Th17, Tc1, Tc17, and Th1/Th17 cells subset levels than men. By contrast, only TEM Th1/Th17 and TEM Tc17 in PBMC differed between males and females. CONCLUSION: Sex-based differences observed in the gut T-cell response of HIV-1-infected patients might contribute to the disease dimorphism.

Increased IL-17 and/or IFN-γ producing T-cell subsets in gut mucosa of long-term-treated HIV-1-infected women / D'Ettorre, Gabriella; Borrazzo, Cristian; Pinacchio, Claudia; Santinelli, Letizia; Cavallari, Eugenio N; Statzu, Maura; Fanello, Gianfranco; Ceccarelli, Giancarlo; Antonelli, Guido; Vullo, Vincenzo; Mastroianni, Claudio M; Scagnolari, Carolina. - In: AIDS. - ISSN 0269-9370. - 33:4(2019), pp. 627-636. [10.1097/QAD.0000000000002122]

Increased IL-17 and/or IFN-γ producing T-cell subsets in gut mucosa of long-term-treated HIV-1-infected women

d'Ettorre, Gabriella
Primo
;
Borrazzo, Cristian;Pinacchio, Claudia;Santinelli, Letizia;Cavallari, Eugenio N;Statzu, Maura;Fanello, Gianfranco;Ceccarelli, Giancarlo;Antonelli, Guido;Vullo, Vincenzo;Mastroianni, Claudio M
Penultimo
;
Scagnolari, Carolina
Ultimo
2019

Abstract

OBJECTIVE: The influence of sex on gut mucosal T-cell response in HIV-1 infection remains largely unknown. We explored whether the frequencies of interferon-γ and/or IL-17 producing naive, T central memory and T effector memory (TEM) CD4 (Th1, Th17) and CD8 T (Tc1, Tc17) cells measured in gut and peripheral districts differed between female and male HIV-1-infected patients. METHODS: Thirty long-term-treated HIV-1-infected individuals were enrolled. The frequencies of Th1, Th17, Tc1, Tc17-cell subsets (single and double) were evaluated by multiparametric flow cytometry in lamina propria lymphocytes and peripheral blood mononuclear cells (PBMC). RESULTS: A sex-based pattern was recorded in the differences of Th1, Th17, Tc1, Tc17-cell subset (single and double) frequencies between gut and peripheral blood. Female patients had stronger alterations in the gut mucosal T-cell repertoire, especially increased Th1, Th17, and Th1/Th17-cell subset frequencies, compared with the blood district than their male counterparts. Higher naive Tc1, Tc17, Tc1/Tc17, TEM Tc17, and TEM Tc1/Tc17 levels were also recorded in the gut mucosa than in the PBMC of HIV-1-infected women. Males and females also differed in their gut T-cell response, with women being characterized by higher Th1, Th17, Tc1, Tc17, and Th1/Th17 cells subset levels than men. By contrast, only TEM Th1/Th17 and TEM Tc17 in PBMC differed between males and females. CONCLUSION: Sex-based differences observed in the gut T-cell response of HIV-1-infected patients might contribute to the disease dimorphism.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/1243734
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