Extracellular-released vesicles (EVs), such as microvesicles (MV) and exosomes (Exo) provide a new type of inter-cellular communication, directly transferring a ready to use box of information, consisting of proteins, lipids and nucleic acids. In the nervous system, EVs participate to neuron-glial cross-talk, a bidirectional communication important to preserve brain homeostasis and, when dysfunctional, involved in several CNS diseases. We investigated whether microglia-derived EVs could be used to transfer a protective phenotype to dysfunctional microglia in the context of a brain tumor. When MV, isolated from microglia stimulated with LPS/IFNg were brain injected in glioma-bearing mice, we observed a phenotype switch of tumor associated myeloid cells (TAMs) and a reduction of tumor size. Our findings indicate that the MV cargo, which contains upregulated transcripts for several inflammation-related genes, can transfer information in the brain of glioma bearing mice modifying microglial gene expression, reducing neuronal death and glioma invasion, thus promoting the recovery of brain homeostasis.

Microglia-derived microvesicles affect microglia phenotype in glioma / Grimaldi, Alfonso; Serpe, Carmela; Chece, Giuseppina; Nigro, Valentina; Sarra, Angelo; Ruzicka, Barbara; Relucenti, Michela; Familiari, Giuseppe; Ruocco, Giancarlo; Pascucci, Giuseppe Rubens; Guerrieri, Francesca; Limatola, Cristina; Catalano, Myriam. - In: FRONTIERS IN CELLULAR NEUROSCIENCE. - ISSN 1662-5102. - 13:(2019), pp. 1-14. [10.3389/fncel.2019.00041]

Microglia-derived microvesicles affect microglia phenotype in glioma

Grimaldi, Alfonso;SERPE, Carmela;Chece, Giuseppina;Nigro, Valentina;Ruzicka, Barbara;Relucenti, Michela;Familiari, Giuseppe;Ruocco, Giancarlo;Limatola, Cristina
;
Catalano, Myriam
2019

Abstract

Extracellular-released vesicles (EVs), such as microvesicles (MV) and exosomes (Exo) provide a new type of inter-cellular communication, directly transferring a ready to use box of information, consisting of proteins, lipids and nucleic acids. In the nervous system, EVs participate to neuron-glial cross-talk, a bidirectional communication important to preserve brain homeostasis and, when dysfunctional, involved in several CNS diseases. We investigated whether microglia-derived EVs could be used to transfer a protective phenotype to dysfunctional microglia in the context of a brain tumor. When MV, isolated from microglia stimulated with LPS/IFNg were brain injected in glioma-bearing mice, we observed a phenotype switch of tumor associated myeloid cells (TAMs) and a reduction of tumor size. Our findings indicate that the MV cargo, which contains upregulated transcripts for several inflammation-related genes, can transfer information in the brain of glioma bearing mice modifying microglial gene expression, reducing neuronal death and glioma invasion, thus promoting the recovery of brain homeostasis.
2019
microglia; extracellular vesicles; tumor associated myeloid cells; brain tumors; glioma
01 Pubblicazione su rivista::01a Articolo in rivista
Microglia-derived microvesicles affect microglia phenotype in glioma / Grimaldi, Alfonso; Serpe, Carmela; Chece, Giuseppina; Nigro, Valentina; Sarra, Angelo; Ruzicka, Barbara; Relucenti, Michela; Familiari, Giuseppe; Ruocco, Giancarlo; Pascucci, Giuseppe Rubens; Guerrieri, Francesca; Limatola, Cristina; Catalano, Myriam. - In: FRONTIERS IN CELLULAR NEUROSCIENCE. - ISSN 1662-5102. - 13:(2019), pp. 1-14. [10.3389/fncel.2019.00041]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1239819
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