We previously demonstrated that bcl-2 over-expression increases the malignant behaviour of the MCF7 ADR human breast cancer cell line and enhances nuclear factor-kappa B (NF-kappaB) transcriptional activity. Here, we investigated the direct effect of increased NF-kappaB activity on the tumorigenicity of MCF7 ADR cells by over-expressing the NF-kappaB subunit relA/p65. Surprisingly, our results demonstrated that over-expression of relA determines a considerable reduction of the tumorigenic ability in nude mice as indicated by the tumour take and the median time of tumour appearance. In vitro studies also evidenced a reduced cell proliferation and the activation of the apoptotic programme after relA over-expression. Apoptosis was associated with the production of reactive oxygen species, and the cleavage of the specific substrate Poly-ADP-ribose-polymerrase. Our data indicate that there is no general role for NF-kappaB in the regulation of apoptosis and tumorigenicity. In fact, even though inhibiting NF-kappaB activity has been reported to be lethal to tumour cells, our findings clearly suggest that an over-induction of nuclear NF-kappaB activity may produce the same effect. (C) 2001 Cancer Research Campaign.
relA over-expression reduces tumorigenicity and activates apoptosis in human cancer cells / A., Ricca; A., Biroccio; D., Trisciuoglio; Cippitelli, Marco; G., Zupi; D., Del Bufalo. - In: BRITISH JOURNAL OF CANCER. - ISSN 0007-0920. - STAMPA. - 85:12(2001), pp. 1914-1921. [10.1054/bjoc.2001.2174]
relA over-expression reduces tumorigenicity and activates apoptosis in human cancer cells
CIPPITELLI, Marco;
2001
Abstract
We previously demonstrated that bcl-2 over-expression increases the malignant behaviour of the MCF7 ADR human breast cancer cell line and enhances nuclear factor-kappa B (NF-kappaB) transcriptional activity. Here, we investigated the direct effect of increased NF-kappaB activity on the tumorigenicity of MCF7 ADR cells by over-expressing the NF-kappaB subunit relA/p65. Surprisingly, our results demonstrated that over-expression of relA determines a considerable reduction of the tumorigenic ability in nude mice as indicated by the tumour take and the median time of tumour appearance. In vitro studies also evidenced a reduced cell proliferation and the activation of the apoptotic programme after relA over-expression. Apoptosis was associated with the production of reactive oxygen species, and the cleavage of the specific substrate Poly-ADP-ribose-polymerrase. Our data indicate that there is no general role for NF-kappaB in the regulation of apoptosis and tumorigenicity. In fact, even though inhibiting NF-kappaB activity has been reported to be lethal to tumour cells, our findings clearly suggest that an over-induction of nuclear NF-kappaB activity may produce the same effect. (C) 2001 Cancer Research Campaign.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.