Nuclear hormone receptors comprise a large family of zinc finger transcription factors, some with hydrophobic Ligands, such as thyroid hormone, vitamin D, steroids, etc., and others for which no ligand has been found. Thyroid hormone receptors (TRs) generally are considered to be confined to the vertebrata that possess a thyroid gland, Tunicates represent the most primitive of the chordates, and there are data supporting a role for thyroid hormone in their metamorphosis, but no data are available on TRs in this genus; hence, we have studied Ciona intestinalis. Screening of a Ciona library with the DNA binding domain of Xenopus laevis TR (xTR) resulted in the isolation of a nuclear hormone receptor, C. intestinalis nuclear receptor 1 (CiNR1), CiNR1 is similar to TRs of more evolved species with a conserved DNA binding domain whereas the ligand binding domain shows poor homology to vertebrate sequences. The C-terminal part of CiNR1 spans approximate to 200 amino acids more than other TRs, lacks the AF2 transactivation domain, and is not able to bind triiodothyronine, Phylogenetically, CiNR1 appears to be close to the common ancestral gene of TRs. Expression of CiNR1 was limited to the developing embryo and the larval stage, which suggests a role during development and metamorphosis, In transfection experiments, CiNR1 down-regulated basal transcription of a reporter gene driven by the TR palindrome responsive element. When CiNR1 was cotransfected with chicken TR alpha, it attenuated the normal thyroid hormone response in a dominant negative fashion. This attenuation required the C-terminal portion of the molecule.

Ciona Intestinalis nuclear receptor 1: a member of the steroid/thyroid hormone receptor family / Carosa, E; Fanelli, A; Ulisse, Salvatore; DI LAURO, R; Rall, Je; Jannini, Ea. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 95:(1998), pp. 1152-1157. [10.1073/pnas.95.19.11152]

Ciona Intestinalis nuclear receptor 1: a member of the steroid/thyroid hormone receptor family.

ULISSE, SALVATORE;
1998

Abstract

Nuclear hormone receptors comprise a large family of zinc finger transcription factors, some with hydrophobic Ligands, such as thyroid hormone, vitamin D, steroids, etc., and others for which no ligand has been found. Thyroid hormone receptors (TRs) generally are considered to be confined to the vertebrata that possess a thyroid gland, Tunicates represent the most primitive of the chordates, and there are data supporting a role for thyroid hormone in their metamorphosis, but no data are available on TRs in this genus; hence, we have studied Ciona intestinalis. Screening of a Ciona library with the DNA binding domain of Xenopus laevis TR (xTR) resulted in the isolation of a nuclear hormone receptor, C. intestinalis nuclear receptor 1 (CiNR1), CiNR1 is similar to TRs of more evolved species with a conserved DNA binding domain whereas the ligand binding domain shows poor homology to vertebrate sequences. The C-terminal part of CiNR1 spans approximate to 200 amino acids more than other TRs, lacks the AF2 transactivation domain, and is not able to bind triiodothyronine, Phylogenetically, CiNR1 appears to be close to the common ancestral gene of TRs. Expression of CiNR1 was limited to the developing embryo and the larval stage, which suggests a role during development and metamorphosis, In transfection experiments, CiNR1 down-regulated basal transcription of a reporter gene driven by the TR palindrome responsive element. When CiNR1 was cotransfected with chicken TR alpha, it attenuated the normal thyroid hormone response in a dominant negative fashion. This attenuation required the C-terminal portion of the molecule.
1998
THYROID-HORMONE, DNA-BINDING, GENE, SUPERFAMILY, PROTEINS, RNA, TRANSCRIPTION, ORGANIZATION, FLOUNDER, CLONING
01 Pubblicazione su rivista::01a Articolo in rivista
Ciona Intestinalis nuclear receptor 1: a member of the steroid/thyroid hormone receptor family / Carosa, E; Fanelli, A; Ulisse, Salvatore; DI LAURO, R; Rall, Je; Jannini, Ea. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 95:(1998), pp. 1152-1157. [10.1073/pnas.95.19.11152]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/123811
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