Spermatogenesis has been intensely studied in rodents but remains poorly understood in humans. Here, we used single-cell RNA sequencing to analyze human testes. Clustering analysis of neonatal testes reveals several cell subsets, including cell populations with characteristics of primordial germ cells (PGCs) and spermatogonial stem cells (SSCs). In adult testes, we identify four undifferentiated spermatogonia (SPG) clusters, each of which expresses specific marker genes. We identify protein markers for the most primitive SPG state, allowing us to purify this likely SSC-enriched cell subset. We map the timeline of male germ cell development from PGCs through fetal germ cells to differentiating adult SPG stages. We also define somatic cell subsets in both neonatal and adult testes and trace their developmental trajectories. Our data provide a blueprint of the developing human male germline and supporting somatic cells. The PGC-like and SSC markers are candidates to be used for SSC therapy to treat infertility.

The neonatal and adult human testis defined at the single-cell level / Sohni, Abhishek; Tan, Kun; Song, Hye-Won; Burow, Dana; de Rooij, Dirk G.; Laurent, Louise; Hsieh, Tung-Chin; Rabah, Raja; Hammoud, Saher Sue; Vicini, Elena; Wilkinson, Miles F.. - In: CELL REPORTS. - ISSN 2211-1247. - 26:6(2019), pp. 1501-1517.e4. [10.1016/j.celrep.2019.01.045]

The neonatal and adult human testis defined at the single-cell level

Vicini, Elena;
2019

Abstract

Spermatogenesis has been intensely studied in rodents but remains poorly understood in humans. Here, we used single-cell RNA sequencing to analyze human testes. Clustering analysis of neonatal testes reveals several cell subsets, including cell populations with characteristics of primordial germ cells (PGCs) and spermatogonial stem cells (SSCs). In adult testes, we identify four undifferentiated spermatogonia (SPG) clusters, each of which expresses specific marker genes. We identify protein markers for the most primitive SPG state, allowing us to purify this likely SSC-enriched cell subset. We map the timeline of male germ cell development from PGCs through fetal germ cells to differentiating adult SPG stages. We also define somatic cell subsets in both neonatal and adult testes and trace their developmental trajectories. Our data provide a blueprint of the developing human male germline and supporting somatic cells. The PGC-like and SSC markers are candidates to be used for SSC therapy to treat infertility.
2019
germ cells; leydig cells; peritubular myoid cells; primordial germ cells; sertoli cells; single-cell RNA sequencing; spermatogenesis; spermatogonia; spermatogonial stem cells; testes; biochemistry; genetics and molecular biology (all)
01 Pubblicazione su rivista::01a Articolo in rivista
The neonatal and adult human testis defined at the single-cell level / Sohni, Abhishek; Tan, Kun; Song, Hye-Won; Burow, Dana; de Rooij, Dirk G.; Laurent, Louise; Hsieh, Tung-Chin; Rabah, Raja; Hammoud, Saher Sue; Vicini, Elena; Wilkinson, Miles F.. - In: CELL REPORTS. - ISSN 2211-1247. - 26:6(2019), pp. 1501-1517.e4. [10.1016/j.celrep.2019.01.045]
File allegati a questo prodotto
File Dimensione Formato  
sohni_The neonatal_2019.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 4.12 MB
Formato Adobe PDF
4.12 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1230175
Citazioni
  • ???jsp.display-item.citation.pmc??? 113
  • Scopus 178
  • ???jsp.display-item.citation.isi??? 182
social impact