To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. Results Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK- SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. Conclusions MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production

Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production / Abdoli, Asghar; Soleimanjahi, Hoorieh; Jamali, Abbas; Mehrbod, Parvaneh; Gholami, Shima; Kianmehr, Zahra; Feizi, Neda; Saleh, Maryam; Bahrami, Fariborz; Mokhtari-Azad, Talat; Abdoli, Mohsen; Tavassoti Kheiri, Masoumeh. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - 38:6(2016), pp. 941-948. [10.1007/s10529-016-2069-4]

Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production

Neda Feizi
Secondo
;
2016

Abstract

To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. Results Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK- SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. Conclusions MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production
2016
influenza virus; MDCK-SIAT1 cells; MDCK cells; microcarrier; vaccine
01 Pubblicazione su rivista::01a Articolo in rivista
Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production / Abdoli, Asghar; Soleimanjahi, Hoorieh; Jamali, Abbas; Mehrbod, Parvaneh; Gholami, Shima; Kianmehr, Zahra; Feizi, Neda; Saleh, Maryam; Bahrami, Fariborz; Mokhtari-Azad, Talat; Abdoli, Mohsen; Tavassoti Kheiri, Masoumeh. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - 38:6(2016), pp. 941-948. [10.1007/s10529-016-2069-4]
File allegati a questo prodotto
File Dimensione Formato  
Abdoli_MDCK_2016.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 357.87 kB
Formato Adobe PDF
357.87 kB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1224244
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 16
social impact