Herpesviruses are known to manipulate autophagy to optimize their replication, counteract immune response and probably to promote tumourigenesis. This study explored, for the first time, the impact of human herpesvirus (HHV)-6 lytic infection on autophagy and demonstrated that HHV-6A and B (viruses sharing more than 80 % homology) differently affected this cellular process. Indeed, while HHV-6A (GS) infection of HSB2 cells promoted autophagy, HHV-6B (Z29) or the virus isolated from the serum of roseola infantum-affected patient-inhibited autophagy in Molt-3 cells or in PBMCs, respectively. Interestingly, the different behaviour of HHV-6A and B on the autophagic process was accompanied by different effects on endoplasmic reticulum stress, unfolded protein response and cell survival that was more strongly reduced by HHV-6B infection. We hypothesize that the ability to inhibit autophagy displayed by HHV-6B could be due to the fact that it contains gene homologues of those encoding for TRS1; the protein responsible for the block of autophagy by human cytomegalovirus. Understanding how HHV-6A/B infection regulates autophagy could be of particular interest, as it has been recently shown that this virus may be involved in Alzheimer’s disease in which a dysregulation of autophagy may also play a role.

Impact of HHV-6A and HHV-6B lytic infection on autophagy and endoplasmic reticulum stress / Romeo, Maria Anele; Masuelli, Laura; Gaeta, Aurelia; Nazzari, Cristina; Granato, Marisa; GILARDINI MONTANI, MARIA SAVERIA; Faggioni, Alberto; Cirone, Mara. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - (2019).

Impact of HHV-6A and HHV-6B lytic infection on autophagy and endoplasmic reticulum stress

Maria Anele Romeo;Laura Masuelli;Aurelia Gaeta;Cristina Nazzari;Marisa Granato;Maria Saveria Gilardini Montani;Alberto Faggioni
;
Mara Cirone.
2019

Abstract

Herpesviruses are known to manipulate autophagy to optimize their replication, counteract immune response and probably to promote tumourigenesis. This study explored, for the first time, the impact of human herpesvirus (HHV)-6 lytic infection on autophagy and demonstrated that HHV-6A and B (viruses sharing more than 80 % homology) differently affected this cellular process. Indeed, while HHV-6A (GS) infection of HSB2 cells promoted autophagy, HHV-6B (Z29) or the virus isolated from the serum of roseola infantum-affected patient-inhibited autophagy in Molt-3 cells or in PBMCs, respectively. Interestingly, the different behaviour of HHV-6A and B on the autophagic process was accompanied by different effects on endoplasmic reticulum stress, unfolded protein response and cell survival that was more strongly reduced by HHV-6B infection. We hypothesize that the ability to inhibit autophagy displayed by HHV-6B could be due to the fact that it contains gene homologues of those encoding for TRS1; the protein responsible for the block of autophagy by human cytomegalovirus. Understanding how HHV-6A/B infection regulates autophagy could be of particular interest, as it has been recently shown that this virus may be involved in Alzheimer’s disease in which a dysregulation of autophagy may also play a role.
2019
HHV-6; autophagy; ER stress; UPR; BIP; CHOP
01 Pubblicazione su rivista::01a Articolo in rivista
Impact of HHV-6A and HHV-6B lytic infection on autophagy and endoplasmic reticulum stress / Romeo, Maria Anele; Masuelli, Laura; Gaeta, Aurelia; Nazzari, Cristina; Granato, Marisa; GILARDINI MONTANI, MARIA SAVERIA; Faggioni, Alberto; Cirone, Mara. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - (2019).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1212285
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