An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our droplet illumination readily achieves more than 50% extinction of the light distributed across the Bessel side lobes, providing a more efficient energy localization without loss in transverse resolution. In a standard light sheet fluorescence microscope, we demonstrate a two-fold contrast enhancement imaging micron-scale fluorescent beads. Results pave the way to new opportunities for rapid and deep in vivo observations of large-scale biological systems.

Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy / Di Domenico, Giuseppe; Ruocco, Giancarlo; Colosi, Cristina; Delre, Eugenio; Antonacci, Giuseppe. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018), p. 17178. [10.1038/s41598-018-35006-1]

Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy

Di Domenico, Giuseppe;Ruocco, Giancarlo;DelRe, Eugenio;
2018

Abstract

An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our droplet illumination readily achieves more than 50% extinction of the light distributed across the Bessel side lobes, providing a more efficient energy localization without loss in transverse resolution. In a standard light sheet fluorescence microscope, we demonstrate a two-fold contrast enhancement imaging micron-scale fluorescent beads. Results pave the way to new opportunities for rapid and deep in vivo observations of large-scale biological systems.
2018
Multidisciplinary
01 Pubblicazione su rivista::01a Articolo in rivista
Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy / Di Domenico, Giuseppe; Ruocco, Giancarlo; Colosi, Cristina; Delre, Eugenio; Antonacci, Giuseppe. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018), p. 17178. [10.1038/s41598-018-35006-1]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1209648
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