The work describes the chromatographic separation optimization of polar lipids on Kinetex-EVO, particularly focusing on sulfolipids in spirulina microalgae (Arthrospira platensis). Gradient shape and mobile-phase modifiers (pH and buffer) were tested on lipid standards. Different conditions were evaluated, and resolution, peak capacity, and peak shape were calculated both in negative mode, for sulfolipids and phospholipids, and in positive mode, for glycolipids. A high-confidence lipid identification strategy was also applied. In collaboration with software creators and developers, Lipostar was implemented to improve the identification of phosphoglycerolipids and to allow the identification of glycosylmonoradyl- and glycosyldiradyl-glycerols classes, the last being the main focus of this work. By this approach, an untargeted screening also for searching lipids not yet reported in the literature could be accomplished. The optimized chromatographic conditions and database search were tested for lipid identification first on the standard mixture, then on the polar lipid extract of spirulina microalgae, for which 205 lipids were identified.
Delving into the polar lipidome by optimized chromatographic separation, high-resolution mass spectrometry, and comprehensive identification with lipostar: microalgae as case study / La Barbera, Giorgia; Antonelli, Michela; Cavaliere, Chiara; Cruciani, Gabriele; Goracci, Laura; Montone, Carmela Maria; Piovesana, Susy; Laganà, Aldo; Capriotti, Anna Laura. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - 90:20(2018), pp. 12230-12238. [10.1021/acs.analchem.8b03482]
Delving into the polar lipidome by optimized chromatographic separation, high-resolution mass spectrometry, and comprehensive identification with lipostar: microalgae as case study
La Barbera, Giorgia;Antonelli, Michela;Cavaliere, Chiara;Montone, Carmela Maria;Piovesana, Susy
;Laganà, Aldo;Capriotti, Anna Laura
2018
Abstract
The work describes the chromatographic separation optimization of polar lipids on Kinetex-EVO, particularly focusing on sulfolipids in spirulina microalgae (Arthrospira platensis). Gradient shape and mobile-phase modifiers (pH and buffer) were tested on lipid standards. Different conditions were evaluated, and resolution, peak capacity, and peak shape were calculated both in negative mode, for sulfolipids and phospholipids, and in positive mode, for glycolipids. A high-confidence lipid identification strategy was also applied. In collaboration with software creators and developers, Lipostar was implemented to improve the identification of phosphoglycerolipids and to allow the identification of glycosylmonoradyl- and glycosyldiradyl-glycerols classes, the last being the main focus of this work. By this approach, an untargeted screening also for searching lipids not yet reported in the literature could be accomplished. The optimized chromatographic conditions and database search were tested for lipid identification first on the standard mixture, then on the polar lipid extract of spirulina microalgae, for which 205 lipids were identified.File | Dimensione | Formato | |
---|---|---|---|
LaBarbera_Delving-into_2018.pdf
solo gestori archivio
Tipologia:
Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
324.8 kB
Formato
Adobe PDF
|
324.8 kB | Adobe PDF | Contatta l'autore |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.