Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time. In particular, the proposed detection system shows an estimated limit of detection for the target antibody (anti-human IgG) smaller than 0.19 nM (28 ng/mL), corresponding to a minimum surface mass coverage of 10.3 ng/cm2. Moreover, from the binding curves we successfully derived the equilibrium association and dissociation constants (KA = 7.5 × 107 M−1; KD = 13.26 nM) of the human IgG–anti-human IgG interaction.

Label-free monitoring of human IgG/anti-IgG recognition using Bloch surface waves on 1D photonic crystals / Sinibaldi, Alberto; Occhicone, Agostino; Munzert, Peter; Danz, Norbert; Sonntag, Frank; Michelotti, Francesco. - In: BIOSENSORS. - ISSN 2079-6374. - 8:(2018). [10.3390/bios8030071]

Label-free monitoring of human IgG/anti-IgG recognition using Bloch surface waves on 1D photonic crystals

Sinibaldi, Alberto
Primo
;
Occhicone, Agostino;Michelotti, Francesco
Ultimo
2018

Abstract

Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time. In particular, the proposed detection system shows an estimated limit of detection for the target antibody (anti-human IgG) smaller than 0.19 nM (28 ng/mL), corresponding to a minimum surface mass coverage of 10.3 ng/cm2. Moreover, from the binding curves we successfully derived the equilibrium association and dissociation constants (KA = 7.5 × 107 M−1; KD = 13.26 nM) of the human IgG–anti-human IgG interaction.
Bloch surface waves; Human IgG screening; One-dimensional photonic crystals; Optical biosensors; Antibodies; Biosensing Techniques; Humans; Immunoglobulin G; Nanostructures; Optics and Photonics; Photons; Clinical Biochemistry
01 Pubblicazione su rivista::01a Articolo in rivista
Label-free monitoring of human IgG/anti-IgG recognition using Bloch surface waves on 1D photonic crystals / Sinibaldi, Alberto; Occhicone, Agostino; Munzert, Peter; Danz, Norbert; Sonntag, Frank; Michelotti, Francesco. - In: BIOSENSORS. - ISSN 2079-6374. - 8:(2018). [10.3390/bios8030071]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1194185
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