Efficacy of synthesized bioconjugate of hydrophilic gold nanoparticles (Au 3MPS) loaded MTX was evaluated by topical applicaction on imiquimod-induced psoriasis like mice model by comparing the epidermal thickness and cell proliferation after treatment with nanoparticle alone versus the bioconjugate. Using 50 mg of 5% Imiquimod on shaved back of 8 weeks C57BL/6 mice model for 6 days resulted psoriasis like inflammation. Mice were divided to 4 groups, topically treated with bio-conjugate or nanoparticle alone for 24 or 48 hours and on day 7 or 8 erythema and scaling were scored from 0 to 4. Mice were euthanized and biopsy to evaluate epidermal thickness by H&E-stain and CD3, CD8 &Ki-67 by immunohistochemical stain. The epidermal thickness was significantly reduced in group treated by the conjugate than by nanoparticle alone. It was 0.322μm ±SEM 0.038 vs 0.707μm ±SEM 0.097 and p<0.001. We noticed reduction in the immunopositive Ki-67 cells with significant p<0.001, plus the decrease in CD3 in MTX -Au3MPS treated group compared to the Au 3MPS treated mice after 24h &48 h in epidermis respectively P<0.05, P<0.001 and in dermis 24h &48 h respectively P<0.001, P<0.05. The decrease in CD8 lymphocyte count after 24h &48 h in epidermis was significant P<0.001, P<0.01 and in dermis 24h &48 h respectively P<0.05, P<0.001. After systematic literature review, this is the first study assessing those parameters in psoriasis model under the cutaneous effect of bio-conjugate of MTX and gold nanoparticles. In addition, the data may contribute to clarify the possibility of enhancing the topical delivery of methotrexate by the aid of gold nanoparticle delivery.

Histopathological and immunohistochemical findings in aldara psoriatic mice model after topical application of methotrexate loaded gold nanoparticle: a comparative study / Bessar, Hagar; Venditti, Iole; Fratoddi, Ilaria; Benassi, Luisa; Botti, Elisabetta; Testa, Giuseppe; Vaschieri, Cristina; Shawki, Sameh; Costanzo, Aantonio; Pellacani, Giovanni. - In: JOURNAL OF INVESTIGATIVE DERMATOLOGY. - ISSN 0022-202X. - 136:9(2016), pp. S201-S201. ((Intervento presentato al convegno Annual Meeting of the European-Society-for-Dermatological-Research (ESDR) tenutosi a Munich, Germany [10.1016/j.jid.2016.06.256].

Histopathological and immunohistochemical findings in aldara psoriatic mice model after topical application of methotrexate loaded gold nanoparticle: a comparative study

Fratoddi, Ilaria;Botti, Elisabetta;Testa, Giuseppe;Pellacani, Giovanni
2016

Abstract

Efficacy of synthesized bioconjugate of hydrophilic gold nanoparticles (Au 3MPS) loaded MTX was evaluated by topical applicaction on imiquimod-induced psoriasis like mice model by comparing the epidermal thickness and cell proliferation after treatment with nanoparticle alone versus the bioconjugate. Using 50 mg of 5% Imiquimod on shaved back of 8 weeks C57BL/6 mice model for 6 days resulted psoriasis like inflammation. Mice were divided to 4 groups, topically treated with bio-conjugate or nanoparticle alone for 24 or 48 hours and on day 7 or 8 erythema and scaling were scored from 0 to 4. Mice were euthanized and biopsy to evaluate epidermal thickness by H&E-stain and CD3, CD8 &Ki-67 by immunohistochemical stain. The epidermal thickness was significantly reduced in group treated by the conjugate than by nanoparticle alone. It was 0.322μm ±SEM 0.038 vs 0.707μm ±SEM 0.097 and p<0.001. We noticed reduction in the immunopositive Ki-67 cells with significant p<0.001, plus the decrease in CD3 in MTX -Au3MPS treated group compared to the Au 3MPS treated mice after 24h &48 h in epidermis respectively P<0.05, P<0.001 and in dermis 24h &48 h respectively P<0.001, P<0.05. The decrease in CD8 lymphocyte count after 24h &48 h in epidermis was significant P<0.001, P<0.01 and in dermis 24h &48 h respectively P<0.05, P<0.001. After systematic literature review, this is the first study assessing those parameters in psoriasis model under the cutaneous effect of bio-conjugate of MTX and gold nanoparticles. In addition, the data may contribute to clarify the possibility of enhancing the topical delivery of methotrexate by the aid of gold nanoparticle delivery.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/1190832
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