The two membrane fluorescent probes 2-dimethylamino-6-lauroyl-naphthalene (Laurdan) and 2-dimethylamino-6-propionyl-naphthalene (Prodan) have been used to study the molecular basis of the damage induced in phospholipid membranes by ionizing radiation. Laurdan and Prodan display a spectral sensitivity to the polarity of their environment, showing a red shift of both excitation and emission spectra with increase of the polarity of their environment. Owing to their chemical differences, the two probes are anchored in the membrane with different strengths. In aqueous environments Laurdan is not fluorescent while Prodan shows appreciable fluorescence. Laurdan and Prodan show an opposite response to oxidative damage produced in phospholipid bilayers by ionizing radiation. The results support the model recently developed of water penetration in the bilayer as a consequence of oxidative damage.

Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation / T., Parasassi; Giusti, Anna Maria; E., Gratton; Monaco, Edoardo; M., Raimondi; G. RAVAGNAN AND O., Sapora. - In: REVIEW OF SCIENTIFIC INSTRUMENTS. - ISSN 0034-6748. - STAMPA. - 65(1994), pp. 329-334. [10.1080/09553009414550391]

Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation.

GIUSTI, Anna Maria;MONACO, Edoardo;
1994

Abstract

The two membrane fluorescent probes 2-dimethylamino-6-lauroyl-naphthalene (Laurdan) and 2-dimethylamino-6-propionyl-naphthalene (Prodan) have been used to study the molecular basis of the damage induced in phospholipid membranes by ionizing radiation. Laurdan and Prodan display a spectral sensitivity to the polarity of their environment, showing a red shift of both excitation and emission spectra with increase of the polarity of their environment. Owing to their chemical differences, the two probes are anchored in the membrane with different strengths. In aqueous environments Laurdan is not fluorescent while Prodan shows appreciable fluorescence. Laurdan and Prodan show an opposite response to oxidative damage produced in phospholipid bilayers by ionizing radiation. The results support the model recently developed of water penetration in the bilayer as a consequence of oxidative damage.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/114908
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